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PMID: 92012 Published · ppublish English Journal Article

New procedure for the production of influenza virus-specific double-stranded DNA's.

Nucleic acids research ·Vol. 7 ·No. 6 ·1979-11-24 ·Pages 1445-56

Caton AJ, Robertson JS

Abstract

A novel technique is described for the production of pure, full-length influenza virus ds DNA's corresponding to each segment of the influenza virus genome, and suitable for molecular cloning and restriction endonuclease mapping. The method involves the synthesis of DNA complementary to both virion (negative strand) and messenger (positive strand) RNA, gel purification and annealing. By avoiding the use of SI nuclease, which often removes the terminal regions of DNA duplexes, the method allows transcription of the total sequence information of influenza virion and messenger RNA's into a ds DNA form.

MeSH Terms
Avian Myeloblastosis Virus/enzymology DNA, Single-Stranded DNA, Viral/biosynthesis Genes, Viral Methods Nucleic Acid Hybridization Orthomyxoviridae/metabolism RNA, Messenger/metabolism RNA-Directed DNA Polymerase
Chemicals
DNA, Single-Stranded DNA, Viral RNA, Messenger RNA-Directed DNA Polymerase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Caton A J
Robertson J S
References (27)
27 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/7.6.1445
Published
1979-11-24
Pages
1445-56
Language
English
Region
England
NLM ID
0411011
PMCID
PMC342319
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/7.6.1445 PMC Full Text View journal details
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