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PMID: 9207002 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Requirements for CD1d recognition by human invariant Valpha24+ CD4-CD8- T cells.

The Journal of experimental medicine ·Vol. 186 ·No. 1 ·1997-07-07 ·Pages 109-20

Exley M, Garcia J, Balk SP, Porcelli S

Abstract

A subset of human CD4-CD8- T cells that expresses an invariant Valpha24-JalphaQ T cell receptor (TCR)-alpha chain, paired predominantly with Vbeta11, has been identified. A series of these Valpha24 Vbeta11 clones were shown to have TCR-beta CDR3 diversity and express the natural killer (NK) locus-encoded C-type lectins NKR-P1A, CD94, and CD69. However, in contrast to NK cells, they did not express killer inhibitory receptors, CD16, CD56, or CD57. All invariant Valpha24(+) clones recognized the MHC class I-like CD16 molecule and discriminated between CD1d and other closely related human CD1 proteins, indicating that recognition was TCR-mediated. Recognition was not dependent upon an endosomal targeting motif in the cytoplasmic tail of CD1d. Upon activation by anti-CD3 or CD1d, the clones produced both Th1 and Th2 cytokines. These results demonstrate that human invariant Valpha24+ CD4-CD8- T cells, and presumably the homologous murine NK1+ T cell population, are CD1d reactive and functionally distinct from NK cells. The conservation of this cell population and of the CD1d ligand across species indicates an important immunological function.

MeSH Terms
Animals Antigens, CD/immunology Cytokines/biosynthesis,immunology Flow Cytometry Humans Immunophenotyping Major Histocompatibility Complex/immunology Mice Receptors, Antigen, T-Cell, alpha-beta/genetics,immunology T-Lymphocyte Subsets/immunology
Chemicals
Antigens, CD Cytokines Receptors, Antigen, T-Cell, alpha-beta
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Exley M
Cancer Biology Program, Hematology/Oncology Division, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, Massachusetts 02215, USA.
Garcia J
Balk S P
Porcelli S
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.186.1.109
Published
1997-07-07
Pages
109-20
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2198960
Subset
IM
Grants
NIAID NIH HHS · R01 AI40135 · United States
NIAID NIH HHS · R01-AI33911 · United States
External Links
PubMed source DOI: 10.1084/jem.186.1.109 PMC Full Text View journal details
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