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PMID: 9212441 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

PCR-mediated detection of acidophilic, bioleaching-associated bacteria.

Applied and environmental microbiology ·Vol. 63 ·No. 7 ·1997-07-00 ·Pages 2944-8

De Wulf-Durand P, Bryant LJ, Sly LI

Abstract

The detection of acidophilic microorganisms from mining environments by culture methods is time consuming and unreliable. Several PCR approaches were developed to amplify small-subunit rRNA sequences from the DNA of six bacterial phylotypes associated with acidic mining environments, permitting the detection of the target DNA at concentrations as low as 10 fg.

MeSH Terms

Bacteria/genetics,isolation & purification DNA, Bacterial/analysis,isolation & purification Mining Molecular Sequence Data Oligonucleotide Probes/genetics Phylogeny Polymerase Chain Reaction/methods RNA, Ribosomal, 16S/genetics Sensitivity and Specificity Soil Microbiology

Chemicals

DNA, Bacterial Oligonucleotide Probes RNA, Ribosomal, 16S

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

De Wulf-Durand P

Department of Microbiology, University of Queensland, Brisbane, Australia.

Bryant L J

Sly L I

References (15)

15 references, click to expand

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Article Info

Journal

Applied and environmental microbiology

Abbr.

Appl Environ Microbiol

ISSN

0099-2240

DOI

10.1128/aem.63.7.2944-2948.1997

Published

1997-07-00

Pages

2944-8

Language

English

Region

United States

NLM ID

7605801

PMCID

PMC168590

Subset

Databases

GENBANK

Y11595, Y11596

External Links

PubMed source DOI: 10.1128/aem.63.7.2944-2948.1997 PMC Full Text View journal details

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