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PMID: 9233819 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

A viral sequence in the 3'-untranslated region mimics a 5' cap in facilitating translation of uncapped mRNA.

The EMBO journal ·Vol. 16 ·No. 13 ·1997-07-01 ·Pages 4107-16

Wang S, Browning KS, Miller WA

Abstract

For recognition by the translational machinery, most eukaryotic cellular mRNAs have a 5' cap structure [e.g. m7G(5')ppp(5')N]. We describe a translation enhancer sequence (3'TE) located in the 3'-untranslated region (UTR) of the genome of the PAV barley yellow dwarf virus (BYDV-PAV) which stimulates translation from uncapped mRNA by 30- to 100-fold in vitro and in vivo to a level equal to that of efficient capped mRNAs. A four base duplication within the 3'TE destroyed the stimulatory activity. Efficient translation was recovered by addition of a 5' cap to this mRNA. Translation of both uncapped mRNA containing the 3'TE in cis and capped mRNA lacking any BYDV-PAV sequence was inhibited specifically by added 3'TE RNA in trans. This inhibition was reversed by adding initiation factor 4F (eIF4F), suggesting that the 3'TE, like the 5' cap, mediates eIF4F-dependent translation initiation. The BYDV-PAV 5'UTR was necessary for the 3'TE to function, except when the 3'TE itself was moved to the 5'UTR. Thus, the 3'TE is sufficient for recruiting the translation factors and ribosomes, while the viral 5'UTR may serve only for the long distance 3'-5' communication. Models are proposed to explain this novel mechanism of cap-independent translation initiation facilitated by the 3'UTR.

MeSH Terms
Conserved Sequence Eukaryotic Initiation Factor-4F Genome, Viral Introns Luteovirus/genetics Peptide Initiation Factors/metabolism,pharmacology Protein Biosynthesis RNA Caps RNA, Messenger/metabolism RNA, Viral/metabolism
Chemicals
Eukaryotic Initiation Factor-4F Peptide Initiation Factors RNA Caps RNA, Messenger RNA, Viral
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Wang S
Molecular, Cellular and Developmental Biology Program, Iowa State University, Ames 50011, USA.
Browning K S
Miller W A
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
DOI
10.1093/emboj/16.13.4107
Published
1997-07-01
Pages
4107-16
Language
English
Region
England
NLM ID
8208664
PMCID
PMC1170033
Subset
IM
External Links
PubMed source DOI: 10.1093/emboj/16.13.4107 PMC Full Text View journal details
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