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PMID: 9234719 Published · ppublish English Journal Article

A transcriptional mediator protein that is required for activation of many RNA polymerase II promoters and is conserved from yeast to humans.

Molecular and cellular biology ·Vol. 17 ·No. 8 ·1997-08-00 ·Pages 4622-32

Lee YC, Min S, Gim BS, Kim YJ

Abstract

A temperature-sensitive mutation was obtained in Med6p, a component of the mediator complex from the yeast Saccharomyces cerevisiae. The mediator complex has been shown to enable transcriptional activation in vitro. This mutation in Med6p abolished activation of transcription from four of five inducible promoters tested in vivo. There was no effect, however, on uninduced transcription, transcription of constitutively expressed genes, or transcription by RNA polymerases I and III. Mediator-RNA polymerase II complex isolated from the mutant yeast strain was temperature sensitive for transcriptional activation in a reconstituted in vitro system due to a defect in initiation complex formation. A database search revealed the existence of MED6-related genes in humans and Caenorhabditis elegans, suggesting that the role of mediator in transcriptional activation is conserved throughout the evolution.

MeSH Terms
Amino Acid Sequence Carrier Proteins/genetics Cell Extracts Cell Nucleus/metabolism Cloning, Molecular Coenzymes DNA-Directed RNA Polymerases/metabolism Fungal Proteins/genetics,metabolism,physiology Gene Expression Regulation, Fungal/physiology Genes, Fungal/genetics Humans Mating Factor Mediator Complex Membrane Transport Proteins Molecular Sequence Data Mutation Peptide Biosynthesis Peptides Plant Proteins/genetics Promoter Regions, Genetic/genetics RNA Polymerase II/metabolism Saccharomyces cerevisiae/genetics,metabolism Saccharomyces cerevisiae Proteins Trans-Activators Transcriptional Activation/physiology
Chemicals
Carrier Proteins Cell Extracts Coenzymes Fungal Proteins MED6 protein, S cerevisiae MED6 protein, human Mediator Complex Membrane Transport Proteins Peptides Plant Proteins Saccharomyces cerevisiae Proteins Trans-Activators sucrose transport protein, plant Mating Factor RNA Polymerase II DNA-Directed RNA Polymerases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Lee Y C
Center for Basic Research, Samsung Biomedical Research Institute, Kangnam-ku, Seoul, Korea.
Min S
Gim B S
Kim Y J
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.17.8.4622
Published
1997-08-00
Pages
4622-32
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC232315
Subset
IM
Databases
GENBANK
U78080, U78082
External Links
PubMed source DOI: 10.1128/MCB.17.8.4622 PMC Full Text View journal details
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