Home LiteratureArticle Details
PMID: 9250663 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Suppressors of YCK-encoded yeast casein kinase 1 deficiency define the four subunits of a novel clathrin AP-like complex.

The EMBO journal ·Vol. 16 ·No. 14 ·1997-07-16 ·Pages 4194-204

Panek HR, Stepp JD, Engle HM, Marks KM, Tan PK, Lemmon SK, Robinson LC

Abstract

In Saccharomyces cerevisiae, the redundant YCK1 and YCK2 genes (Yeast Casein Kinase 1) are required for viability. We describe here the molecular analysis of four mutations that eliminate the requirement for Yck activity. These mutations alter proteins that resemble the four subunits of clathrin adaptors (APs), with highest sequence similarity to those of the recently identified AP-3 complex. The four yeast subunits are associated in a high-molecular-weight complex. These proteins have no essential function and are not redundant for function with other yeast AP-related proteins. Combination of suppressor mutations with a clathrin heavy chain mutation (chc1-ts) confers no synthetic growth defects. However, a yck(ts) mutation shows a strong synthetic growth defect with chc1-ts. Moreover, endocytosis of Ste3p is dramatically decreased in yck(ts) cells and is partially restored by the AP suppressor mutations. These results suggest that vesicle trafficking at the plasma membrane requires the activity of Yck protein kinases, and that the new AP-related complex may participate in this process.

MeSH Terms
Adaptor Proteins, Vesicular Transport Blotting, Western Casein Kinase I Casein Kinases Cell Division Clathrin/genetics,metabolism DNA Mutational Analysis Endocytosis Endosomes/metabolism Membrane Proteins/metabolism Molecular Sequence Data Monomeric Clathrin Assembly Proteins Morphogenesis Nerve Tissue Proteins/chemistry,genetics,metabolism Phosphoproteins/chemistry,genetics,metabolism Phosphorylation Protein Conformation Protein Kinases/genetics,metabolism Receptors, Cell Surface/metabolism Receptors, G-Protein-Coupled Receptors, Mating Factor Receptors, Pheromone Saccharomyces cerevisiae/enzymology,genetics,growth & development,metabolism Saccharomyces cerevisiae Proteins Suppression, Genetic/genetics
Chemicals
Adaptor Proteins, Vesicular Transport Clathrin Membrane Proteins Monomeric Clathrin Assembly Proteins Nerve Tissue Proteins Phosphoproteins Receptors, Cell Surface Receptors, G-Protein-Coupled Receptors, Mating Factor Receptors, Pheromone STE3 protein, S cerevisiae Saccharomyces cerevisiae Proteins clathrin assembly protein AP180 Protein Kinases Casein Kinase I Casein Kinases YCK1 protein, S cerevisiae YCK2 protein, S cerevisiae
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Panek H R
LSU Medical Center, Department of Biochemistry and Molecular Biology, Shreveport, LA 71130, USA.
Stepp J D
Engle H M
Marks K M
Tan P K
Lemmon S K
Robinson L C
References (40)
40 references, click to expand
  1. Cytosolic and membrane-associated proteins involved in the recruitment of AP-1 adaptors onto the trans-Golgi network.
    J Biol Chem. 1996 Oct 11;271(41):25446-51 PMID: 8810314
  2. Ubiquitination of the yeast a-factor receptor.
    J Cell Biol. 1996 Aug;134(3):661-74 PMID: 8707846
  3. In vivo phosphorylation of adaptors regulates their interaction with clathrin.
    J Cell Biol. 1996 Nov;135(3):635-45 PMID: 8909539
  4. Chs1p and Chs3p, two proteins involved in chitin synthesis, populate a compartment of the Saccharomyces cerevisiae endocytic pathway.
    Mol Biol Cell. 1996 Dec;7(12):1909-19 PMID: 8970154
  5. Characterization of the adaptor-related protein complex, AP-3.
    J Cell Biol. 1997 May 19;137(4):835-45 PMID: 9151686
  6. The 50 kDa protein subunit of assembly polypeptide (AP) AP-2 adaptor from clathrin-coated vesicles is phosphorylated on threonine-156 by AP-1 and a soluble AP50 kinase which co-purifies with the assembly polypeptides.
    Biochem J. 1993 Dec 1;296 ( Pt 2):409-15 PMID: 8257432
  7. The Saccharomyces cerevisiae APS1 gene encodes a homolog of the small subunit of the mammalian clathrin AP-1 complex: evidence for functional interaction with clathrin at the Golgi complex.
    EMBO J. 1994 Apr 1;13(7):1706-17 PMID: 8157009
  8. A prenylation motif is required for plasma membrane localization and biochemical function of casein kinase I in budding yeast.
    J Biol Chem. 1994 Jul 29;269(30):19271-8 PMID: 8034689
  9. A late Golgi sorting function for Saccharomyces cerevisiae Apm1p, but not for Apm2p, a second yeast clathrin AP medium chain-related protein.
    Mol Biol Cell. 1995 Jan;6(1):41-58 PMID: 7749194
  10. Casein kinase I gamma subfamily. Molecular cloning, expression, and characterization of three mammalian isoforms and complementation of defects in the Saccharomyces cerevisiae YCK genes.
    J Biol Chem. 1995 May 26;270(21):12717-24 PMID: 7759525
  11. Transformation of intact yeast cells treated with alkali cations.
    J Bacteriol. 1983 Jan;153(1):163-8 PMID: 6336730
  12. Antisuppressor mutation in Escherichia coli defective in biosynthesis of 5-methylaminomethyl-2-thiouridine.
    J Bacteriol. 1985 Jan;161(1):368-76 PMID: 3881393
  13. A ten-minute DNA preparation from yeast efficiently releases autonomous plasmids for transformation of Escherichia coli.
    Gene. 1987;57(2-3):267-72 PMID: 3319781
  14. In vivo phosphorylation of clathrin-coated vesicle proteins from rat reticulocytes.
    J Biol Chem. 1988 Mar 25;263(9):4408-15 PMID: 2894378
  15. Control of glycogen synthase by hierarchal protein phosphorylation.
    FASEB J. 1990 Sep;4(12):2961-8 PMID: 2168324
  16. Basic local alignment search tool.
    J Mol Biol. 1990 Oct 5;215(3):403-10 PMID: 2231712
  17. Identification of a putative yeast homolog of the mammalian beta chains of the clathrin-associated protein complexes.
    Mol Cell Biol. 1990 Nov;10(11):6089-90 PMID: 2122239
  18. Casein kinase I and II--multipotential serine protein kinases: structure, function, and regulation.
    Adv Second Messenger Phosphoprotein Res. 1991;23:123-64 PMID: 1997039
  19. AP17 and AP19, the mammalian small chains of the clathrin-associated protein complexes show homology to Yap17p, their putative homolog in yeast.
    J Biol Chem. 1991 Jun 15;266(17):11153-7 PMID: 2040623
  20. HRR25, a putative protein kinase from budding yeast: association with repair of damaged DNA.
    Science. 1991 Aug 30;253(5023):1031-4 PMID: 1887218
  21. Purification of casein kinase I and isolation of cDNAs encoding multiple casein kinase I-like enzymes.
    Proc Natl Acad Sci U S A. 1991 Nov 1;88(21):9548-52 PMID: 1946367
  22. The medium chains of the mammalian clathrin-associated proteins have a homolog in yeast.
    Eur J Biochem. 1991 Dec 5;202(2):569-74 PMID: 1761056
  23. Two genes in Saccharomyces cerevisiae encode a membrane-bound form of casein kinase-1.
    Mol Biol Cell. 1992 Mar;3(3):275-86 PMID: 1627830
  24. Selective and immediate effects of clathrin heavy chain mutations on Golgi membrane protein retention in Saccharomyces cerevisiae.
    J Cell Biol. 1992 Aug;118(3):531-40 PMID: 1322413
  25. Cell cycle-dependent localization of casein kinase I to mitotic spindles.
    Proc Natl Acad Sci U S A. 1992 Oct 15;89(20):9454-8 PMID: 1409656
  26. Yeast pheromone receptor endocytosis and hyperphosphorylation are independent of G protein-mediated signal transduction.
    Cell. 1992 Nov 27;71(5):755-63 PMID: 1330324
  27. Molecular cloning, expression, and characterization of a 49-kilodalton casein kinase I isoform from rat testis.
    J Biol Chem. 1993 Mar 25;268(9):6394-401 PMID: 8454611
  28. Casein kinase I-like protein kinases encoded by YCK1 and YCK2 are required for yeast morphogenesis.
    Mol Cell Biol. 1993 May;13(5):2870-81 PMID: 8474447
  29. The binding of AP-1 clathrin adaptor particles to Golgi membranes requires ADP-ribosylation factor, a small GTP-binding protein.
    Cell. 1993 Jun 4;73(5):999-1005 PMID: 8500185
  30. Cis- and trans-acting functions required for endocytosis of the yeast pheromone receptors.
    J Cell Biol. 1993 Jul;122(1):53-65 PMID: 8391002
  31. Isolation and properties of YCK2, a Saccharomyces cerevisiae homolog of casein kinase-1.
    Arch Biochem Biophys. 1993 Aug 15;305(1):47-53 PMID: 8342955
  32. Biochemical dissection of AP-1 recruitment onto Golgi membranes.
    J Cell Biol. 1993 Nov;123(3):561-73 PMID: 8227126
  33. Isolation and characterization of human casein kinase I epsilon (CKI), a novel member of the CKI gene family.
    J Biol Chem. 1995 Jun 23;270(25):14875-83 PMID: 7797465
  34. Beta-NAP, a cerebellar degeneration antigen, is a neuron-specific vesicle coat protein.
    Cell. 1995 Sep 8;82(5):773-83 PMID: 7671305
  35. Targeting signals and subunit interactions in coated vesicle adaptor complexes.
    J Cell Biol. 1995 Nov;131(3):619-30 PMID: 7593184
  36. The ARF1 GTPase-activating protein: zinc finger motif and Golgi complex localization.
    Science. 1995 Dec 22;270(5244):1999-2002 PMID: 8533093
  37. Ubiquitination of a yeast plasma membrane receptor signals its ligand-stimulated endocytosis.
    Cell. 1996 Jan 26;84(2):277-87 PMID: 8565073
  38. A novel adaptor-related protein complex.
    J Cell Biol. 1996 May;133(4):749-60 PMID: 8666661
  39. Cloning, expression pattern and mapping to 12p 13.2 --> p13.1 of CLAPS3, a gene encoding a novel clathrin-adaptor small chain.
    Cytogenet Cell Genet. 1996;73(3):214-7 PMID: 8697810
  40. Prenylated isoforms of yeast casein kinase I, including the novel Yck3p, suppress the gcs1 blockage of cell proliferation from stationary phase.
    Mol Cell Biol. 1996 Oct;16(10):5375-85 PMID: 8816449
Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
DOI
10.1093/emboj/16.14.4194
Published
1997-07-16
Pages
4194-204
Language
English
Region
England
NLM ID
8208664
PMCID
PMC1170045
Subset
IM
Grants
PHS HHS · 39040 · United States
NIA NIH HHS · AG00105 · United States
Databases
GENBANK
G07072, L07073, L07074, L13939, L26291, M23674, M34177, M62419, M64998, R02669, R54523, T30164, T98538, U09841, U35411, U36858, U37673, U44890, X54424, X60288, X76053, X91067, Z36157, Z49274, Z50113, Z71255
SWISSPROT
P18484, P36000, P38065
External Links
PubMed source DOI: 10.1093/emboj/16.14.4194 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2