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PMID: 9258684 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Induction of duplication reversion in human fibroblasts, by wild-type and mutated SV40 T antigen, covaries with the ability to induce host DNA synthesis.

Genetics ·Vol. 146 ·No. 4 ·1997-08-00 ·Pages 1417-28

Shammas MA, Xia SJ, Shmookler Reis RJ

Abstract

Intrachromosomal homologous recombination, manifest as reversion of a 14-kbp duplication in the hypoxanthine phosphoribosyl transferase (HPRT) gene, is elevated in human cells either stably transformed or transiently transfected by the SV40 (simian virus 40) large T antigen gene. Following introduction of wild-type SV40, or any of several T-antigen point mutations in a constant SV40 background, we observed a strong correlation between the stimulation of chromosomal recombination and induction of host-cell DNA synthesis. Moreover, inhibitors of DNA replication (aphidicolin and hydroxyurea) suppress SV40-induced homologous recombination to the extent that they suppress DNA synthesis. Stable integration of plasmids encoding T antigen also augments homologous recombination, which is suppressed by aphidicolin. We infer that the mechanism by which T antigen stimulates homologous recombination in human fibroblasts involves DNA replicative synthesis.

MeSH Terms
Antigens, Polyomavirus Transforming/genetics Aphidicolin/pharmacology Cell Line Cell Transformation, Neoplastic/genetics DNA/biosynthesis DNA Replication/drug effects,genetics Fibroblasts Genes, Viral Humans Hydroxyurea/pharmacology Hypoxanthine Phosphoribosyltransferase/genetics Multigene Family Mutation Recombination, Genetic Simian virus 40/genetics Transfection Transformation, Genetic Viral Structural Proteins/genetics
Chemicals
Antigens, Polyomavirus Transforming Viral Structural Proteins Aphidicolin DNA Hypoxanthine Phosphoribosyltransferase Hydroxyurea
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Shammas M A
Department of Biochemistry, University of Arkansas for Medical Sciences, Little Rock 72205, USA.
Xia S J
Shmookler Reis R J
References (49)
49 references, click to expand
  1. Oncogenes result in genomic alterations that activate a transcriptionally silent, dominantly selectable reporter gene (neo).
    Mol Cell Biol. 1992 Jan;12(1):198-206 PMID: 1309588
  2. Interaction of simian virus 40 large T-antigen with cellular DNA polymerase alpha: studies with various T-antigen mutants of SV40.
    Arch Virol. 1991;118(1-2):113-25 PMID: 1646591
  3. Structure and function of simian virus 40 large tumor antigen.
    Annu Rev Biochem. 1992;61:55-85 PMID: 1323237
  4. Functional characterization of temperature-sensitive mutants of simian virus 40 large T antigen.
    J Virol. 1992 Nov;66(11):6509-16 PMID: 1328677
  5. An interaction between replication protein A and SV40 T antigen appears essential for primosome assembly during SV40 DNA replication.
    J Biol Chem. 1993 Feb 15;268(5):3389-95 PMID: 8381428
  6. DNA polymerase alpha stimulates the ATP-dependent binding of simian virus tumor T antigen to the SV40 origin of replication.
    J Biol Chem. 1993 May 25;268(15):11018-27 PMID: 8388381
  7. Subunit rearrangement of the cyclin-dependent kinases is associated with cellular transformation.
    Genes Dev. 1993 Aug;7(8):1572-83 PMID: 8101826
  8. Modulation of cell growth, p34cdc2 and cyclin A levels by SV-40 large T antigen.
    Oncogene. 1993 Nov;8(11):2987-93 PMID: 8414501
  9. Homologous recombination of monkey alpha-satellite repeats in an in vitro simian virus 40 replication system: possible association of recombination with DNA replication.
    Mol Cell Biol. 1994 Jun;14(6):4173-82 PMID: 8196655
  10. The kinetics of simian virus 40-induced progression of quiescent cells into S phase depend on four independent functions of large T antigen.
    J Virol. 1994 Sep;68(9):5496-508 PMID: 8057432
  11. Recombination by replication.
    Cell. 1996 May 31;85(5):625-7 PMID: 8646771
  12. Role of postreplicative DNA mismatch repair in the cytotoxic action of thioguanine.
    Science. 1996 Aug 23;273(5278):1109-11 PMID: 8688098
  13. Differential interaction of temperature-sensitive simian virus 40 T antigens with tumor suppressors pRb and p53.
    J Virol. 1996 Oct;70(10):7224-7 PMID: 8794371
  14. The helix-loop-helix protein Id-1 and a retinoblastoma protein binding mutant of SV40 T antigen synergize to reactivate DNA synthesis in senescent human fibroblasts.
    Dev Genet. 1996;18(2):161-72 PMID: 8934878
  15. Effect of fractionated doses of radiation on recombination.
    Heredity (Edinb). 1968 Feb;23(1):143-6 PMID: 5245620
  16. Effect of hydroxyurea on ribonucleotide reductase.
    Biochem Biophys Res Commun. 1968 Oct 10;33(1):129-35 PMID: 4301391
  17. Radiation-induced recombination in Saccharomyces: the genetic control of recombination in mitosis and meiosis.
    Radiat Res. 1972 Jan;49(1):148-54 PMID: 4550515
  18. DNA infectivity and the induction of host DNA synthesis with temperature-sensitive mutants of simian virus 40.
    J Virol. 1975 Jan;15(1):145-50 PMID: 163339
  19. Comparison by autoradiography of macromolecular biosynthesis in "young" and "old" human diploid fibroblast cultures. A brief note.
    Mech Ageing Dev. 1977 Sep-Oct;6(5):379-84 PMID: 895211
  20. Evidence that spontaneous mitotic recombination occurs at the two-strand stage.
    Proc Natl Acad Sci U S A. 1978 Sep;75(9):4436-40 PMID: 360220
  21. Separation of lytic and transforming functions of the simian virus 40 A region: two mutants which are temperature sensitive for lytic functions have opposite effects on transformation.
    J Virol. 1981 May;38(2):518-28 PMID: 6264125
  22. Chromosomal rearrangement and carcinogenesis.
    Mutat Res. 1982 May;98(3):249-64 PMID: 7050694
  23. DNA binding properties of simian virus 40 temperature-sensitive A proteins.
    J Virol. 1982 Nov;44(2):458-66 PMID: 6292510
  24. Cellular oncogenes and multistep carcinogenesis.
    Science. 1983 Nov 18;222(4625):771-8 PMID: 6356358
  25. Oligomerization of simian virus 40 large T antigen is not necessarily repressed by temperature-sensitive A gene lesions.
    J Virol. 1984 Mar;49(3):658-64 PMID: 6321754
  26. Transgenic mice harboring SV40 T-antigen genes develop characteristic brain tumors.
    Cell. 1984 Jun;37(2):367-79 PMID: 6327063
  27. [Possible role of the T antigen in inducing chromosome aberrations in SV40 virus-transformed cells].
    Tsitologiia. 1985 Jan;27(1):76-82 PMID: 2984814
  28. Recombination events after transient infection and stable integration of DNA into mouse cells.
    Mol Cell Biol. 1985 Apr;5(4):659-66 PMID: 3990687
  29. Heritable formation of pancreatic beta-cell tumours in transgenic mice expressing recombinant insulin/simian virus 40 oncogenes.
    Nature. 1985 May 9-15;315(6015):115-22 PMID: 2986015
  30. Reinitiation of cellular DNA synthesis in BrdU-selected nondividing senescent WI-38 cells by simian virus 40 infection.
    J Cell Physiol. 1985 Oct;125(1):122-6 PMID: 2995423
  31. The SV40 large T-p53 complex: evidence for the presence of two immunologically distinct forms of p53.
    Virology. 1985 Nov;147(1):206-9 PMID: 2998064
  32. RNase H-defective mutants of Escherichia coli.
    J Bacteriol. 1986 May;166(2):361-3 PMID: 3009391
  33. Synthesis of linear plasmid multimers in Escherichia coli K-12.
    J Bacteriol. 1986 Jul;167(1):327-35 PMID: 3522548
  34. Homologous recombination between coinjected DNA sequences peaks in early to mid-S phase.
    Mol Cell Biol. 1987 Jun;7(6):2294-5 PMID: 3600663
  35. Generation of linear multigenome-length plasmid molecules in Bacillus subtilis.
    Nucleic Acids Res. 1987 Aug 25;15(16):6349-67 PMID: 3114717
  36. Unwinding of duplex DNA from the SV40 origin of replication by T antigen.
    Science. 1987 Nov 13;238(4829):964-7 PMID: 2823389
  37. Spontaneous reversion of novel Lesch-Nyhan mutation by HPRT gene rearrangement.
    Somat Cell Mol Genet. 1988 May;14(3):293-303 PMID: 2835825
  38. Localized melting and structural changes in the SV40 origin of replication induced by T-antigen.
    EMBO J. 1988 Oct;7(10):3149-58 PMID: 2846276
  39. Spontaneous rearrangement of integrated simian virus 40 DNA in nine transformed rodent cell lines.
    J Virol. 1989 Jan;63(1):165-74 PMID: 2562813
  40. Enhanced transfection efficiency and improved cell survival after electroporation of G2/M-synchronized cells and treatment with sodium butyrate.
    Nucleic Acids Res. 1989 May 25;17(10):3959-71 PMID: 2786626
  41. Temperature-sensitive mutants identify crucial structural regions of simian virus 40 large T antigen.
    J Virol. 1989 Oct;63(10):4426-30 PMID: 2778883
  42. Reversible cellular senescence: implications for immortalization of normal human diploid fibroblasts.
    Mol Cell Biol. 1989 Jul;9(7):3088-92 PMID: 2779554
  43. Homologous plasmid recombination is elevated in immortally transformed cells.
    Mol Cell Biol. 1989 Sep;9(9):4009-17 PMID: 2550810
  44. Three domains in the simian virus 40 core origin orchestrate the binding, melting, and DNA helicase activities of T antigen.
    J Virol. 1990 Feb;64(2):509-18 PMID: 2153220
  45. SV40 T antigen alone drives karyotype instability that precedes neoplastic transformation of human diploid fibroblasts.
    J Cell Biochem. 1990 Jan;42(1):13-31 PMID: 2153691
  46. Interconversion of replication and recombination structures: implications for terminal repeats and concatemers.
    J Theor Biol. 1990 May 22;144(2):195-202 PMID: 2165201
  47. Interactions between SV40 T antigen and DNA polymerase alpha.
    New Biol. 1990 Jan;2(1):84-92 PMID: 1964085
  48. The zinc finger region of simian virus 40 large T antigen is needed for hexamer assembly and origin melting.
    J Virol. 1991 Jun;65(6):3167-74 PMID: 1851875
  49. Effect of aphidicolin on DNA synthesis, PLD-recovery and DNA repair of human diploid fibroblasts.
    Int J Radiat Biol. 1992 Feb;61(2):191-7 PMID: 1351906
Article Info
Journal
Genetics
Abbr.
Genetics
ISSN
0016-6731
DOI
10.1093/genetics/146.4.1417
Published
1997-08-00
Pages
1417-28
Language
English
Region
United States
NLM ID
0374636
PMCID
PMC1208085
Subset
IM
External Links
PubMed source DOI: 10.1093/genetics/146.4.1417 PMC Full Text View journal details
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