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PMID: 9279389 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

No activation of new initiation points for deoxyribonucleic acid replication in BALB/c 3T3 cells transformed by Kirsten sarcoma virus.

Molecular and cellular biology ·Vol. 1 ·No. 8 ·1981-08-00 ·Pages 763-8

Oppenheim A, Horowitz AT

Abstract

BALB/c 3T3 cells were transformed by Kirsten sarcoma virus, and five clones were isolated in soft agar. Average replicon sizes of the transformed cell lines were estimated by the method of fiber-autoradiography (J. A. Huberman and A. D. Riggs, J. Mol. Biol.32:327-341, 1968) and found to be the same size as the nontransformed 3T3 cells, analyzed in parallel. The results indicate that, unlike simian virus 40 and Epstein-Barr virus, Kirsten sarcoma virus does not activate new initiation points for cellular deoxyribonucleic acid replication in murine sarcoma virus-transformed BALB/c 3T3 cells.

MeSH Terms
3T3 Cells Animals Autoradiography Cell Transformation, Viral/genetics DNA Replication DNA, Viral/biosynthesis Kirsten murine sarcoma virus/genetics Mice
Chemicals
DNA, Viral
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Oppenheim A
Chanock Center for Virology, Hebrew University-Hadassah Medical School, Jerusalem, Israel.
Horowitz A T
References (32)
32 references, click to expand
  1. On the mechanism of DNA replication in mammalian chromosomes.
    J Mol Biol. 1968 Mar 14;32(2):327-41 PMID: 5689363
  2. AGAR SUSPENSION CULTURE FOR THE SELECTIVE ASSAY OF CELLS TRANSFORMED BY POLYOMA VIRUS.
    Virology. 1964 Jun;23:291-4 PMID: 14187925
  3. Adaptation of plaque assay methods to the in vitro quantitation of the radiation leukemia virus.
    J Virol. 1973 Jul;12(1):68-73 PMID: 4353501
  4. Initiation of DNA replication in mammalian cells and its inhibition by reovirus infection.
    J Mol Biol. 1974 Jan 15;82(2):175-83 PMID: 4361361
  5. The units of DNA replication in Drosophila melanogaster chromosomes.
    Cold Spring Harb Symp Quant Biol. 1974;38:205-23 PMID: 4208784
  6. Viral DNA synthesis in cells infected by temperature-sensitive mutants of simian virus 40.
    J Virol. 1974 Jul;14(1):116-24 PMID: 4366021
  7. Interaction of partially purified simian virus 40 T antigen with circular viral DNA molecules.
    Proc Natl Acad Sci U S A. 1975 May;72(5):1960-4 PMID: 168585
  8. Characterization of Epstein-Barr virus antigens. I. Biochemical analysis of the complement-fixing soluble antigen and relationship with Epstein-Barr virus-associated nuclear antigen.
    J Virol. 1976 Feb;17(2):672-4 PMID: 176436
  9. Solubilization of the Epstein-Barr virus-determined nuclear antigen and its characterization as a DNA-binding protein.
    J Virol. 1977 Apr;22(1):1-8 PMID: 192908
  10. Identification of a purified complement-fixing antigen as the Epstein-Barr-virus determined nuclear antigen (EBNA) by its binding to metaphase chromosomes.
    Proc Natl Acad Sci U S A. 1977 Apr;74(4):1605-9 PMID: 67603
  11. Initiation points for DNA replication in nontransformed and simian virus 40-transformed Chinese hamster lung cells.
    Cell. 1977 Aug;11(4):859-69 PMID: 196764
  12. Polypeptides of cells transformed by RNA or DNA tumor viruses.
    Proc Natl Acad Sci U S A. 1977 Jul;74(7):2729-33 PMID: 197517
  13. Interaction of Simian Virus 40 chromatin with Simian Virus 40 T-antigen.
    J Virol. 1977 Nov;24(2):451-60 PMID: 199733
  14. Detection of a nuclear, EBNA-type antigen in apparently EBNA-negative Herpesvirus papio (HVP)-transformed lymphoid lines by the acid-fixed nuclear binding technique.
    Int J Cancer. 1977 Dec 15;20(6):941-6 PMID: 73523
  15. The binding site on SV40 DNA for a T antigen-related protein.
    Cell. 1978 Jan;13(1):165-79 PMID: 202398
  16. Initiation points for DNA replication in nontransformed and simian virus 40-transformed BALB/c 3T3 cells.
    J Virol. 1978 Jan;25(1):450-52 PMID: 202755
  17. Protein kinase activity associated with the avian sarcoma virus src gene product.
    Proc Natl Acad Sci U S A. 1978 Apr;75(4):2021-4 PMID: 205879
  18. Identification of a polypeptide encoded by the avian sarcoma virus src gene.
    Proc Natl Acad Sci U S A. 1978 Mar;75(3):1567-71 PMID: 206909
  19. Simian virus 40 T antigen binds to host cell chromosomes.
    Nature. 1978 Jul 13;274(5667):164-6 PMID: 208006
  20. Evidence that the transforming gene of avian sarcoma virus encodes a protein kinase associated with a phosphoprotein.
    Cell. 1978 Oct;15(2):561-72 PMID: 214242
  21. Enzymatic activities associated with a purified simian virus 40 T antigen-related protein.
    Proc Natl Acad Sci U S A. 1979 Feb;76(2):610-4 PMID: 218212
  22. Detection of a nuclear antigen in Herpesvirus ateles-carrying marmoset lines by the acid-fixed nuclear binding technique.
    Proc Natl Acad Sci U S A. 1979 Apr;76(4):2042-6 PMID: 221913
  23. Identification of a sarcoma virus-coded phosphoprotein in nonproducer cells transformed by Kirsten or Harvey murine sarcoma virus.
    Virology. 1979 Jul 15;96(1):64-79 PMID: 223311
  24. p21 of Kirsten murine sarcoma virus is thermolabile in a viral mutant temperature sensitive for the maintenance of transformation.
    J Virol. 1979 Aug;31(2):546-6 PMID: 225570
  25. Guanine nucleotide-binding activity as an assay for src protein of rat-derived murine sarcoma viruses.
    Proc Natl Acad Sci U S A. 1979 Oct;76(10):5355-9 PMID: 228288
  26. Localization of the ASV src gene product to the plasma membrane of transformed cells by electron microscopic immunocytochemistry.
    Cell. 1979 Sep;18(1):125-34 PMID: 228858
  27. Avian sarcoma virus-transforming protein, pp60src shows protein kinase activity specific for tyrosine.
    Nature. 1980 May 15;285(5761):167-9 PMID: 6246443
  28. Transforming gene product of Rous sarcoma virus phosphorylates tyrosine.
    Proc Natl Acad Sci U S A. 1980 Mar;77(3):1311-5 PMID: 6246487
  29. Localization of the src gene product of the Harvey strain of MSV to plasma membrane of transformed cells by electron microscopic immunocytochemistry.
    Cell. 1980 Apr;19(4):1005-14 PMID: 6247068
  30. Initiation points for cellular deoxyribonucleic acid replication in human lymphoid cells converted by Epstein-Barr virus.
    Mol Cell Biol. 1981 Aug;1(8):753-62 PMID: 9279388
  31. Characteristics of an assay for Rous sarcoma virus and Rous sarcoma cells in tissue culture.
    Virology. 1958 Dec;6(3):669-88 PMID: 13616179
  32. Simian virus 40 deoxyribonucleic acid synthesis: the viral replicon.
    J Virol. 1972 Oct;10(4):591-8 PMID: 4343541
Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.1.8.763-768.1981
Published
1981-08-00
Pages
763-8
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC369356
Subset
IM
External Links
PubMed source DOI: 10.1128/mcb.1.8.763-768.1981 PMC Full Text View journal details
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