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PMID: 9293027 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Cross-regulation of toluene monooxygenases by the transcriptional activators TbmR and TbuT.

Applied and environmental microbiology ·Vol. 63 ·No. 9 ·1997-09-00 ·Pages 3736-9

Leahy JG, Johnson GR, Olsen RH

Abstract

The toluene-3-monooxygenase from Burkholderia pickettii PKO1 and the toluene/benzene-2-monooxygenase from Burkholderia (Pseudomonas) sp. strain JS150 are distinct enzymes which differ not only in catalytic specificity and substrate range but also in the arrangement and sequence of the genes within the operons that encode the enzymes, tbuA1UBVA2C and tbmABCDEF, respectively. In the present study, we examined the transcriptional activation of the PtbuA1 and PtbmA promoters by their cognate regulators, TbuT and TbmR. TbmR and TbuT each exhibited activation of both PtbmA and PtbuA1, with toluene, benzene, and chlorobenzene serving as strong effectors. These results strongly suggest that TbmR is an NtrC-like regulator which is functionally homologous to TbuT, and they provide evidence for the evolutionary "recruitment" of the same or a similar type of regulator for both monooxygenase pathways.

MeSH Terms
Base Sequence Biological Evolution Burkholderia/enzymology,genetics DNA Primers/genetics DNA, Bacterial/genetics Gene Expression Regulation, Bacterial/drug effects Gene Expression Regulation, Enzymologic/drug effects Genes, Bacterial/drug effects Molecular Sequence Data Operon/drug effects Oxygenases/genetics,metabolism Promoter Regions, Genetic/drug effects Trans-Activators/genetics,pharmacology Transcriptional Activation/drug effects
Chemicals
DNA Primers DNA, Bacterial Trans-Activators Oxygenases toluene-3-monooxygenase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Leahy J G
Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor 48109-0620, USA.
Johnson G R
Olsen R H
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24 references, click to expand
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Article Info
Journal
Applied and environmental microbiology
Abbr.
Appl Environ Microbiol
ISSN
0099-2240
DOI
10.1128/aem.63.9.3736-3739.1997
Published
1997-09-00
Pages
3736-9
Language
English
Region
United States
NLM ID
7605801
PMCID
PMC168682
Subset
IM
Grants
NIEHS NIH HHS · ES-04911 · United States
NCRR NIH HHS · M01RR00042 · United States
External Links
PubMed source DOI: 10.1128/aem.63.9.3736-3739.1997 PMC Full Text View journal details
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