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PMID: 9294138 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

CCR6, a CC chemokine receptor that interacts with macrophage inflammatory protein 3alpha and is highly expressed in human dendritic cells.

The Journal of experimental medicine ·Vol. 186 ·No. 6 ·1997-09-15 ·Pages 837-44

Greaves DR, Wang W, Dairaghi DJ, Dieu MC, Saint-Vis B, Franz-Bacon K, Rossi D, Caux C, McClanahan T, Gordon S, Zlotnik A, Schall TJ

Abstract

Dendritic cells initiate immune responses by ferrying antigen from the tissues to the lymphoid organs for presentation to lymphocytes. Little is known about the molecular mechanisms underlying this migratory behavior. We have identified a chemokine receptor which appears to be selectively expressed in human dendritic cells derived from CD34+ cord blood precursors, but not in dendritic cells derived from peripheral blood monocytes. When stably expressed as a recombinant protein in a variety of host cell backgrounds, the receptor shows a strong interaction with only one chemokine among 25 tested: the recently reported CC chemokine macrophage inflammatory protein 3alpha. Thus, we have designated this receptor as the CC chemokine receptor 6. The cloning and characterization of a dendritic cell CC chemokine receptor suggests a role for chemokines in the control of the migration of dendritic cells and the regulation of dendritic cell function in immunity and infection.

MeSH Terms
Amino Acid Sequence Animals Base Sequence CHO Cells Chromosome Mapping Cloning, Molecular Cricetinae DNA, Complementary/genetics Dendritic Cells/immunology,metabolism Gene Expression Humans Hybrid Cells Macrophage Inflammatory Proteins/metabolism Molecular Sequence Data Polymerase Chain Reaction RNA, Messenger/genetics,metabolism Receptors, CCR6 Receptors, Chemokine Receptors, Cytokine/genetics,metabolism Recombinant Proteins/genetics,metabolism Sequence Homology, Amino Acid Transfection
Chemicals
CCR6 protein, human DNA, Complementary Macrophage Inflammatory Proteins RNA, Messenger Receptors, CCR6 Receptors, Chemokine Receptors, Cytokine Recombinant Proteins
Authors & Affiliations
12 authors, click to expand affiliations / ORCID
Greaves D R
Sir William Dunn School of Pathology, University of Oxford, Oxford OX1 3RE, United Kingdom.
Wang W
Dairaghi D J
Dieu M C
Saint-Vis B
Franz-Bacon K
Rossi D
Caux C
McClanahan T
Gordon S
Zlotnik A
Schall T J
References (33)
33 references, click to expand
  1. Human blood contains two subsets of dendritic cells, one immunologically mature and the other immature.
    Immunology. 1994 Jul;82(3):487-93 PMID: 7525461
  2. Molecular cloning and RNA expression of two new human chemokine receptor-like genes.
    Biochem Biophys Res Commun. 1996 Oct 23;227(3):846-53 PMID: 8886020
  3. Recent advances in the study of dendritic cells and follicular dendritic cells.
    Immunol Today. 1995 Jan;16(1):2-4 PMID: 7880384
  4. Chemokines, leukocyte trafficking, and inflammation.
    Curr Opin Immunol. 1994 Dec;6(6):865-73 PMID: 7710711
  5. Activation of dual T cell signaling pathways by the chemokine RANTES.
    Science. 1995 Sep 22;269(5231):1727-30 PMID: 7569902
  6. Migration of dendritic cells in response to formyl peptides, C5a, and a distinct set of chemokines.
    J Immunol. 1995 Oct 1;155(7):3292-5 PMID: 7561021
  7. Molecular cloning and functional characterization of a novel member of the C-C chemokine family.
    J Immunol. 1995 Dec 1;155(11):5352-8 PMID: 7594550
  8. Identification of RANTES, MIP-1 alpha, and MIP-1 beta as the major HIV-suppressive factors produced by CD8+ T cells.
    Science. 1995 Dec 15;270(5243):1811-5 PMID: 8525373
  9. HIV-1 entry cofactor: functional cDNA cloning of a seven-transmembrane, G protein-coupled receptor.
    Science. 1996 May 10;272(5263):872-7 PMID: 8629022
  10. HIV-1 Langerhans' cell tropism associated with heterosexual transmission of HIV.
    Science. 1996 Mar 1;271(5253):1291-3 PMID: 8638113
  11. Identification of a major co-receptor for primary isolates of HIV-1.
    Nature. 1996 Jun 20;381(6584):661-6 PMID: 8649511
  12. HIV-1 entry into CD4+ cells is mediated by the chemokine receptor CC-CKR-5.
    Nature. 1996 Jun 20;381(6584):667-73 PMID: 8649512
  13. Chemokine receptors: gateways to inflammation and infection.
    Nat Med. 1996 Nov;2(11):1174-8 PMID: 8898734
  14. CD4-independent infection by HIV-2 is mediated by fusin/CXCR4.
    Cell. 1996 Nov 15;87(4):745-56 PMID: 8929542
  15. Efficient interaction of HIV-1 with purified dendritic cells via multiple chemokine coreceptors.
    J Exp Med. 1996 Dec 1;184(6):2433-8 PMID: 8976200
  16. Identification through bioinformatics of two new macrophage proinflammatory human chemokines: MIP-3alpha and MIP-3beta.
    J Immunol. 1997 Feb 1;158(3):1033-6 PMID: 9013939
  17. A new class of membrane-bound chemokine with a CX3C motif.
    Nature. 1997 Feb 13;385(6617):640-4 PMID: 9024663
  18. Molecular cloning of a novel human CC chemokine liver and activation-regulated chemokine (LARC) expressed in liver. Chemotactic activity for lymphocytes and gene localization on chromosome 2.
    J Biol Chem. 1997 Feb 28;272(9):5846-53 PMID: 9038201
  19. Chemokines: leucocyte recruitment and activation cytokines.
    Lancet. 1997 Feb 15;349(9050):490-5 PMID: 9040590
  20. Cloning of STRL22, a new human gene encoding a G-protein-coupled receptor related to chemokine receptors and located on chromosome 6q27.
    Genomics. 1997 Feb 15;40(1):175-80 PMID: 9070937
  21. Identification of CCR6, the specific receptor for a novel lymphocyte-directed CC chemokine LARC.
    J Biol Chem. 1997 Jun 6;272(23):14893-8 PMID: 9169459
  22. The dendritic cell system and its role in immunogenicity.
    Annu Rev Immunol. 1991;9:271-96 PMID: 1910679
  23. Interactions of tumor necrosis factor with granulocyte-macrophage colony-stimulating factor and other cytokines in the regulation of dendritic cell growth in vitro from early bipotent CD34+ progenitors in human bone marrow.
    J Immunol. 1992 Oct 15;149(8):2681-8 PMID: 1383322
  24. Interleukin-8 and related chemotactic cytokines--CXC and CC chemokines.
    Adv Immunol. 1994;55:97-179 PMID: 8304236
  25. Efficient presentation of soluble antigen by cultured human dendritic cells is maintained by granulocyte/macrophage colony-stimulating factor plus interleukin 4 and downregulated by tumor necrosis factor alpha.
    J Exp Med. 1994 Apr 1;179(4):1109-18 PMID: 8145033
  26. New insights into the mobilization and phagocytic activity of dendritic cells.
    J Exp Med. 1996 Apr 1;183(4):1287-92 PMID: 8666885
  27. The beta-chemokine receptors CCR3 and CCR5 facilitate infection by primary HIV-1 isolates.
    Cell. 1996 Jun 28;85(7):1135-48 PMID: 8674119
  28. Homozygous defect in HIV-1 coreceptor accounts for resistance of some multiply-exposed individuals to HIV-1 infection.
    Cell. 1996 Aug 9;86(3):367-77 PMID: 8756719
  29. CD34+ hematopoietic progenitors from human cord blood differentiate along two independent dendritic cell pathways in response to GM-CSF+TNF alpha.
    J Exp Med. 1996 Aug 1;184(2):695-706 PMID: 8760823
  30. Frequent infection of peripheral blood CD8-positive T-lymphocytes with HIV-1. Edinburgh Heterosexual Transmission Study Group.
    Lancet. 1996 Sep 7;348(9028):649-54 PMID: 8782755
  31. Human recombinant monocyte chemotactic protein and other C-C chemokines bind and induce directional migration of dendritic cells in vitro.
    J Leukoc Biol. 1996 Sep;60(3):365-71 PMID: 8830793
  32. Improved methods for the generation of dendritic cells from nonproliferating progenitors in human blood.
    J Immunol Methods. 1996 Sep 27;196(2):121-35 PMID: 8841451
  33. Three populations of cells with dendritic morphology exist in peripheral blood, only one of which is infectable with human immunodeficiency virus type 1.
    Proc Natl Acad Sci U S A. 1995 Jan 31;92(3):826-30 PMID: 7846060
Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.186.6.837
Published
1997-09-15
Pages
837-44
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2199049
Subset
IM
External Links
PubMed source DOI: 10.1084/jem.186.6.837 PMC Full Text View journal details
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