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PMID: 9315630 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Endothelial cytosolic proteins bind to the 3' untranslated region of endothelial nitric oxide synthase mRNA: regulation by tumor necrosis factor alpha.

Molecular and cellular biology ·Vol. 17 ·No. 10 ·1997-10-00 ·Pages 5719-26

Alonso J, Sánchez de Miguel L, Montón M, Casado S, López-Farré A

Abstract

Changes in endothelial nitric oxide synthase (eNOS) expression may be involved in the endothelium-dependent vasorelaxation dysfunction associated with several vascular diseases. In the present work, we demonstrate that eNOS mRNA contains a previously undescribed cis element in the 3' untranslated region (3' UTR). A U+C-rich segment in the 3' UTR is critical in complex formation with bovine aortic endothelial cell cytosolic proteins. Tumor necrosis factor alpha (TNF-alpha), which destabilizes eNOS mRNA, increased the binding activity of the cytosolic proteins in a time-dependent manner. These data suggest that endothelial cytosolic proteins bind to the 3' UTR of eNOS mRNA. These proteins may play a role in TNF-alpha-induced eNOS mRNA destabilization.

MeSH Terms
Animals Base Sequence Cattle Cells, Cultured Cytosol/chemistry Endothelium, Vascular/chemistry,enzymology Molecular Sequence Data Nitric Oxide Synthase/genetics Polyribonucleotides/metabolism RNA, Messenger/metabolism RNA-Binding Proteins/metabolism Regulatory Sequences, Nucleic Acid/genetics Tumor Necrosis Factor-alpha/pharmacology
Chemicals
Polyribonucleotides RNA, Messenger RNA-Binding Proteins Tumor Necrosis Factor-alpha Nitric Oxide Synthase
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Alonso J
Nephrology, Hypertension and Cardiovascular Research Laboratory, Instituto de Investigaciones Médicas, Fundación Jiménez Díaz, Madrid, Spain.
Sánchez de Miguel L
Montón M
Casado S
López-Farré A
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.17.10.5719
Published
1997-10-00
Pages
5719-26
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC232420
Subset
IM
Corrections
CommentIn
PMID: 12436961
CommentIn
PMID: 12894803
External Links
PubMed source DOI: 10.1128/MCB.17.10.5719 PMC Full Text View journal details
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