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PMID: 9321663 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The spoU gene of Escherichia coli, the fourth gene of the spoT operon, is essential for tRNA (Gm18) 2'-O-methyltransferase activity.

Nucleic acids research ·Vol. 25 ·No. 20 ·1997-10-15 ·Pages 4093-7

Persson BC, Jäger G, Gustafsson C

Abstract

We have evidence that the open reading frame previously denoted spoU is necessary for tRNA (Gm18) 2'-O-methyltransferase activity. The spoU gene is located in the gmk-rpoZ-spoT-spoU-recG operon at 82 minutes on the Escherichia coli chromosome. The deduced amino acid sequence of spoU shows strong similarities to previously characterized 2'-O-methyltransferases. Comparison of the nucleoside modification pattern of hydrolyzed tRNA, 16S rRNA and 23S rRNA from wild-type and spoU null mutants showed that the modified nucleoside 2'-O-methylguanosine (Gm), present in a subset of E. coli tRNAs at residue 18, is completely absent in the spoU mutant, suggesting that spoU encodes tRNA (Gm18) 2'-O-methyltransferase. Nucleoside modification of 16S and 23S rRNA was unaffected in the spoU mutant. Insertions in the downstream recG gene did not affect RNA modification. Absence of Gm18 in tRNA does not influence growth rate under the tested conditions and does not interfere with activity of the SupF amber suppressor, a suppressor tRNA that normally has the Gm18 modification. We suggest that the spoU gene be renamed trmH (tRNA methylation).

MeSH Terms
Amino Acid Sequence Escherichia coli/genetics Genes, Bacterial Genes, Suppressor Operon Pyrophosphatases/genetics RNA, Transfer/genetics RNA, Transfer, Tyr/metabolism Sequence Homology tRNA Methyltransferases/chemistry,genetics,metabolism
Chemicals
RNA, Transfer, Tyr supF tRNA RNA, Transfer tRNA Methyltransferases tRNA (guanine(10)-N(2))-dimethyltransferase guanosine-3',5'-bis(diphosphate) 3'-pyrophosphatase Pyrophosphatases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Persson B C
Department of Microbiology, Umeâ University, S-901 87 Umeâ, Sweden. [email protected]
Jäger G
Gustafsson C
References (30)
30 references, click to expand
  1. Effects of surrounding sequence on the suppression of nonsense codons.
    J Mol Biol. 1983 Feb 15;164(1):59-71 PMID: 6188840
  2. Acetylornithinase of Escherichia coli: partial purification and some properties.
    J Biol Chem. 1956 Jan;218(1):97-106 PMID: 13278318
  3. Nucleotide sequences encoding and promoting expression of three antibiotic resistance genes indigenous to Streptomyces.
    Mol Gen Genet. 1985;199(1):26-36 PMID: 2987648
  4. Structural analysis of RNA using chemical and enzymatic probing monitored by primer extension.
    Methods Enzymol. 1988;164:481-9 PMID: 2468070
  5. Linkage map of Escherichia coli K-12, edition 8.
    Microbiol Rev. 1990 Jun;54(2):130-97 PMID: 2194094
  6. Basic local alignment search tool.
    J Mol Biol. 1990 Oct 5;215(3):403-10 PMID: 2231712
  7. Residual guanosine 3',5'-bispyrophosphate synthetic activity of relA null mutants can be eliminated by spoT null mutations.
    J Biol Chem. 1991 Mar 25;266(9):5980-90 PMID: 2005134
  8. Molecular organization and nucleotide sequence of the recG locus of Escherichia coli K-12.
    J Bacteriol. 1991 Nov;173(21):6837-43 PMID: 1938888
  9. Conformational rigidity of specific pyrimidine residues in tRNA arises from posttranscriptional modifications that enhance steric interaction between the base and the 2'-hydroxyl group.
    Biochemistry. 1992 Feb 4;31(4):1040-6 PMID: 1310418
  10. The nucleotide sequence of recG, the distal spo operon gene in Escherichia coli K-12.
    Gene. 1992 Jan 2;110(1):95-9 PMID: 1544582
  11. Relation between functions and conformational characteristics of modified nucleosides found in tRNAs.
    Nucleic Acids Symp Ser. 1991;(25):49-50 PMID: 1726811
  12. Guanylate kinase of Escherichia coli K-12.
    J Biol Chem. 1993 Jul 5;268(19):14316-21 PMID: 8390989
  13. SpoU protein of Escherichia coli belongs to a new family of putative rRNA methylases.
    Nucleic Acids Res. 1993 Nov 25;21(23):5519 PMID: 8265370
  14. Functional requirement of a site-specific ribose methylation in ribosomal RNA.
    Science. 1993 Dec 17;262(5141):1886-9 PMID: 8266080
  15. Rit1, a tRNA backbone-modifying enzyme that mediates initiator and elongator tRNA discrimination.
    Cell. 1994 Nov 4;79(3):535-46 PMID: 7954819
  16. The cellular factor TRP-185 regulates RNA polymerase II binding to HIV-1 TAR RNA.
    EMBO J. 1995 Dec 1;14(23):5995-6009 PMID: 8846792
  17. Compilation of tRNA sequences and sequences of tRNA genes.
    Nucleic Acids Res. 1996 Jan 1;24(1):68-72 PMID: 8594604
  18. The in vitro synthesis of 2'-omethylguanosine and 2-methylthio 6N (gamma,gamma, dimethylallyl) adenosine in transfer RNA of Escherichia coli.
    Biochem Biophys Res Commun. 1969 Aug 7;36(3):435-41 PMID: 4898378
  19. The effect of growth temperatures on the in vivo ribose methylation of Bacillus stearothermophilus transfer RNA.
    Arch Biochem Biophys. 1973 Jan;154(1):277-82 PMID: 4689778
  20. Culture medium for enterobacteria.
    J Bacteriol. 1974 Sep;119(3):736-47 PMID: 4604283
  21. Structure of yeast phenylalanine transfer RNA at 2.5 A resolution.
    Proc Natl Acad Sci U S A. 1975 Nov;72(11):4414-8 PMID: 1105583
  22. Hydrogen bonding in yeast phenylalanine transfer RNA.
    Proc Natl Acad Sci U S A. 1975 Dec;72(12):4866-70 PMID: 1108007
  23. A thermostable tRNA (guanosine-2')-methyltransferase from Thermus thermophilus HB27 and the effect of ribose methylation on the conformational stability of tRNA.
    J Biol Chem. 1982 Jul 10;257(13):7388-95 PMID: 7085632
  24. Quantitative enzymatic hydrolysis of tRNAs: reversed-phase high-performance liquid chromatography of tRNA nucleosides.
    J Chromatogr. 1982 Jul 9;230(2):297-308 PMID: 7050138
  25. Site-specific ribose methylation of preribosomal RNA: a novel function for small nucleolar RNAs.
    Cell. 1996 Jun 28;85(7):1077-88 PMID: 8674114
  26. Intron-encoded, antisense small nucleolar RNAs: the characterization of nine novel species points to their direct role as guides for the 2'-O-ribose methylation of rRNAs.
    J Mol Biol. 1996 Jul 12;260(2):178-95 PMID: 8764399
  27. Identification of new RNA modifying enzymes by iterative genome search using known modifying enzymes as probes.
    Nucleic Acids Res. 1996 Oct 1;24(19):3756-62 PMID: 8871555
  28. A small nucleolar RNA requirement for site-specific ribose methylation of rRNA in Xenopus.
    Proc Natl Acad Sci U S A. 1996 Dec 10;93(25):14480-5 PMID: 8962077
  29. Studies on lysogenesis. I. The mode of phage liberation by lysogenic Escherichia coli.
    J Bacteriol. 1951 Sep;62(3):293-300 PMID: 14888646
  30. Complete analysis of tRNA-modified nucleosides by high-performance liquid chromatography: the 29 modified nucleosides of Salmonella typhimurium and Escherichia coli tRNA.
    Anal Biochem. 1983 Feb 15;129(1):1-13 PMID: 6190418
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/25.20.4093
Email
[email protected]
Published
1997-10-15
Pages
4093-7
Language
English
Region
England
NLM ID
0411011
PMCID
PMC146995
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/25.20.4093 PMC Full Text View journal details
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