A microplate enzyme-immunoassay for toxoplasma antibody.

Journal of clinical pathology ·Vol. 29 ·No. 2 ·1976-02-00 ·Pages 150-3

Voller A, Bidwell DE, Bartlett A, Fleck DG, Perkins M, Oladehin B

Abstract

A new test for the detection and measurement of toxoplasma antibody is described. Test sera are reacted with antigen-sensitized wells in micro-haemagglutination plates. Any attached antibody is shown by the addition of an enzyme-labelled antiglobulin followed by assay of the enzyme reaction with its substrate. The test is easy to carry out on a large scale, and there is a positive correlation between the results and dye test and haemagglutination test titres.

MeSH Terms

Alkaline Phosphatase Antibodies/analysis Antigen-Antibody Reactions Coloring Agents Hemagglutination Tests Humans Immunoassay/methods Toxoplasmosis/diagnosis

Chemicals

Antibodies Coloring Agents Alkaline Phosphatase

Authors & Affiliations

6 authors, click to expand affiliations / ORCID

Voller A

Bidwell D E

Bartlett A

Fleck D G

Perkins M

Oladehin B

References (6)

6 references, click to expand

Microplate enzyme-linked immunosorbent assay for chagas' disease.
Lancet. 1975 Feb 22;1(7904):426-8 PMID: 48613
The relationship and significance of antibody titres as determined by various serological methods in glandular and ocular toxoplasmosis.
J Clin Pathol. 1975 Jan;28(1):42-9 PMID: 1091663
New serological test for malaria antibodies.
Br Med J. 1975 Mar 22;1(5959):659-61 PMID: 1092412
Enzyme-linked immunosorbent assay, Elisa. 3. Quantitation of specific antibodies by enzyme-labeled anti-immunoglobulin in antigen-coated tubes.
J Immunol. 1972 Jul;109(1):129-35 PMID: 4113792
A hemagglutination test for toxoplasmosis.
J Parasitol. 1957 Jun;43(3):308-14 PMID: 13439469
Dyes as Microchemical Indicators of a New Immunity Phenomenon Affecting a Protozoon Parasite (Toxoplasma).
Science. 1948 Dec 10;108(2815):660-3 PMID: 17744024

Article Info

Journal

Journal of clinical pathology

Abbr.

J Clin Pathol

ISSN

0021-9746

DOI

10.1136/jcp.29.2.150

Published

1976-02-00

Pages

150-3

Language

English

Region

England

NLM ID

0376601

PMCID

PMC475993

Subset

External Links

PubMed source DOI: 10.1136/jcp.29.2.150 PMC Full Text View journal details

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A microplate enzyme-immunoassay for toxoplasma antibody.

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