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PMID: 9327737 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

CXCR-4 (Fusin), a co-receptor for the type 1 human immunodeficiency virus (HIV-1), is expressed in the human brain in a variety of cell types, including microglia and neurons.

The American journal of pathology ·Vol. 151 ·No. 4 ·1997-10-00 ·Pages 1035-42

Lavi E, Strizki JM, Ulrich AM, Zhang W, Fu L, Wang Q, O'Connor M, Hoxie JA, González-Scarano F

Abstract

Entry of the type 1 human immunodeficiency virus into most cells requires the presence of the CD4 protein in combination with one of several recently described co-receptors. CXCR-4 (fusin) was the first identified, and it serves as co-receptor for T-cell-line tropic (T-tropic) HIV-1 isolates. To determine the expression of CXCR-4 in the brain, a major target of HIV pathology, we used immunohistochemistry and reverse transcriptase polymerase chain reaction with CXCR-4-specific antibodies and probes. We found that CXCR-4 was expressed in several cell types in brain, but notably in neurons and microglia, a finding that was replicated in tissue culture. The study of the expression of CXCR-4 in the brain, which may be one of many chemokine receptors in the central nervous system, may provide further insight into the interactions between brain cells, pathogens, and the immune system, and help understand the pathogenesis of HIV dementia.

MeSH Terms
AIDS Dementia Complex/metabolism,pathology Adult Aged Antibodies, Monoclonal Brain/metabolism,pathology Cells, Cultured DNA Primers/chemistry Female HIV-1 Humans Immunohistochemistry In Situ Hybridization Male Microglia/metabolism,pathology Middle Aged Neurons/metabolism,pathology Polymerase Chain Reaction RNA, Messenger/metabolism Receptors, CXCR4/biosynthesis,genetics,immunology
Chemicals
Antibodies, Monoclonal DNA Primers RNA, Messenger Receptors, CXCR4
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Lavi E
Department of Pathology and Laboratory Medicine (Division of Neuropathology), University of Pennsylvania School of Medicine, Philadelphia 19104-6085, USA.
Strizki J M
Ulrich A M
Zhang W
Fu L
Wang Q
O'Connor M
Hoxie J A
González-Scarano F
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Article Info
Journal
The American journal of pathology
Abbr.
Am J Pathol
ISSN
0002-9440
Published
1997-10-00
Pages
1035-42
Language
English
Region
United States
NLM ID
0370502
PMCID
PMC1858037
Subset
IM
Grants
NINDS NIH HHS · NS-27405 · United States
NINDS NIH HHS · NS-30606 · United States
External Links
PubMed source PMC Full Text View journal details
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