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PMID: 9380751 Published · ppublish English Journal Article

hSK4, a member of a novel subfamily of calcium-activated potassium channels.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 94 ·No. 20 ·1997-09-30 ·Pages 11013-8

Joiner WJ, Wang LY, Tang MD, Kaczmarek LK

Abstract

The gene for hSK4, a novel human small conductance calcium-activated potassium channel, or SK channel, has been identified and expressed in Chinese hamster ovary cells. In physiological saline hSK4 generates a conductance of approximately 12 pS, a value in close agreement with that of other cloned SK channels. Like other members of this family, the polypeptide encoded by hSK4 contains a previously unnoted leucine zipper-like domain in its C terminus of unknown function. hSK4 appears unique, however, in its very high affinity for Ca2+ (EC50 of 95 nM) and its predominant expression in nonexcitable tissues of adult animals. Together with the relatively low homology of hSK4 to other SK channel polypeptides (approximately 40% identical), these data suggest that hSK4 belongs to a novel subfamily of SK channels.

MeSH Terms
Amino Acid Sequence Animals Barium/metabolism CHO Cells Calcium/metabolism Cloning, Molecular Cricetinae Humans Intermediate-Conductance Calcium-Activated Potassium Channels Molecular Sequence Data Potassium Channels/genetics,metabolism Potassium Channels, Calcium-Activated Sequence Homology, Amino Acid
Chemicals
Intermediate-Conductance Calcium-Activated Potassium Channels KCNN4 protein, human Potassium Channels Potassium Channels, Calcium-Activated Barium Calcium
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Joiner W J
Department of Cellular and Molecular Physiology, Yale University School of Medicine, 333 Cedar Street, New Haven, CT 06520, USA.
Wang L Y
Tang M D
Kaczmarek L K
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.94.20.11013
Published
1997-09-30
Pages
11013-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC23566
Subset
IM
Databases
GENBANK
AF000972, AF000973
External Links
PubMed source DOI: 10.1073/pnas.94.20.11013 PMC Full Text View journal details
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