Home LiteratureArticle Details
PMID: 9389650 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

sel-10, a negative regulator of lin-12 activity in Caenorhabditis elegans, encodes a member of the CDC4 family of proteins.

Genes & development ·Vol. 11 ·No. 23 ·1997-12-01 ·Pages 3182-93

Hubbard EJ, Wu G, Kitajewski J, Greenwald I

Abstract

Mutations that influence lin-12 activity in Caenorhabditis elegans may identify conserved factors that regulate the activity of lin-12/Notch proteins. We describe genetic evidence indicating that sel-10 is a negative regulator of lin-12/Notch-mediated signaling in C. elegans. Sequence analysis shows that SEL-10 is a member of the CDC4 family of proteins and has a potential human ortholog. Coimmunoprecipitation data indicate that C. elegans SEL-10 complexes with LIN-12 and with murine Notch4. We propose that SEL-10 promotes the ubiquitin-mediated turnover of LIN-12/Notch proteins, and discuss potential roles for the regulation of lin-12/Notch activity by sel-10 in cell fate decisions and tumorigenesis.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Caenorhabditis elegans/genetics,metabolism Caenorhabditis elegans Proteins Cell Cycle Proteins/chemistry,genetics,metabolism Cell Differentiation/physiology Cell Transformation, Neoplastic Cloning, Molecular DNA, Complementary F-Box Proteins F-Box-WD Repeat-Containing Protein 7 Gene Dosage Genes, Helminth Helminth Proteins/chemistry,genetics,metabolism Humans Membrane Proteins/genetics,metabolism Mice Molecular Sequence Data Mutation Proto-Oncogene Proteins/genetics,metabolism Receptor, Notch4 Receptors, Cell Surface Receptors, Notch Sequence Homology, Amino Acid Signal Transduction Ubiquitin-Protein Ligases Ubiquitins/metabolism
Chemicals
Caenorhabditis elegans Proteins Cell Cycle Proteins DNA, Complementary F-Box Proteins F-Box-WD Repeat-Containing Protein 7 FBXW7 protein, human Fbxw7 protein, mouse Helminth Proteins Lin-12 protein, C elegans Membrane Proteins NOTCH4 protein, human Proto-Oncogene Proteins Receptor, Notch4 Receptors, Cell Surface Receptors, Notch SEL-10 protein, C elegans Ubiquitins Notch4 protein, mouse Ubiquitin-Protein Ligases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Hubbard E J
Department of Biochemistry and Molecular Biophysics, Howard Hughes Medical Institute, New York, New York 10032, USA.
Wu G
Kitajewski J
Greenwald I
References (56)
56 references, click to expand
  1. The genetics of Caenorhabditis elegans.
    Genetics. 1974 May;77(1):71-94 PMID: 4366476
  2. The ancient regulatory-protein family of WD-repeat proteins.
    Nature. 1994 Sep 22;371(6495):297-300 PMID: 8090199
  3. Alterations in cell lineage following laser ablation of cells in the somatic gonad of Caenorhabditis elegans.
    Dev Biol. 1981 Oct 30;87(2):286-300 PMID: 7286433
  4. The lin-12 locus specifies cell fates in Caenorhabditis elegans.
    Cell. 1983 Sep;34(2):435-44 PMID: 6616618
  5. The ubiquitin-proteasome proteolytic pathway.
    Cell. 1994 Oct 7;79(1):13-21 PMID: 7923371
  6. Reciprocal changes in expression of the receptor lin-12 and its ligand lag-2 prior to commitment in a C. elegans cell fate decision.
    Cell. 1994 Dec 30;79(7):1187-98 PMID: 8001154
  7. lin-25, a gene required for vulval induction in Caenorhabditis elegans.
    Genes Dev. 1995 Feb 1;9(3):341-57 PMID: 7867931
  8. Recurrent chromosomal translocations and fusion genes in leukemia-lymphoma cell lines.
    Leukemia. 1995 Mar;9(3):480-500 PMID: 7885046
  9. Notch signaling.
    Science. 1995 Apr 14;268(5208):225-32 PMID: 7716513
  10. The sulfur controller-2 negative regulatory gene of Neurospora crassa encodes a protein with beta-transducin repeats.
    Proc Natl Acad Sci U S A. 1995 Apr 11;92(8):3343-7 PMID: 7724564
  11. Primary neurogenesis in Xenopus embryos regulated by a homologue of the Drosophila neurogenic gene Delta.
    Nature. 1995 Jun 29;375(6534):761-6 PMID: 7596407
  12. Met30p, a yeast transcriptional inhibitor that responds to S-adenosylmethionine, is an essential protein with WD40 repeats.
    Mol Cell Biol. 1995 Dec;15(12):6526-34 PMID: 8524217
  13. Vertebrate retinal ganglion cells are selected from competent progenitors by the action of Notch.
    Development. 1995 Nov;121(11):3637-50 PMID: 8582277
  14. The 2.0 A crystal structure of a heterotrimeric G protein.
    Nature. 1996 Jan 25;379(6563):311-9 PMID: 8552184
  15. Crystal structure of a G-protein beta gamma dimer at 2.1A resolution.
    Nature. 1996 Jan 25;379(6563):369-74 PMID: 8552196
  16. Direct and long-range action of a DPP morphogen gradient.
    Cell. 1996 May 3;85(3):357-68 PMID: 8616891
  17. lag-1, a gene required for lin-12 and glp-1 signaling in Caenorhabditis elegans, is homologous to human CBF1 and Drosophila Su(H).
    Development. 1996 May;122(5):1373-83 PMID: 8625826
  18. cul-1 is required for cell cycle exit in C. elegans and identifies a novel gene family.
    Cell. 1996 Jun 14;85(6):829-39 PMID: 8681378
  19. Notch4/int-3, a mammary proto-oncogene, is an endothelial cell-specific mammalian Notch gene.
    Development. 1996 Jul;122(7):2251-9 PMID: 8681805
  20. SKP1 connects cell cycle regulators to the ubiquitin proteolysis machinery through a novel motif, the F-box.
    Cell. 1996 Jul 26;86(2):263-74 PMID: 8706131
  21. A comprehensive set of sequence analysis programs for the VAX.
    Nucleic Acids Res. 1984 Jan 11;12(1 Pt 1):387-95 PMID: 6546423
  22. The structure of an antigenic determinant in a protein.
    Cell. 1984 Jul;37(3):767-78 PMID: 6204768
  23. Isolation of monoclonal antibodies specific for human c-myc proto-oncogene product.
    Mol Cell Biol. 1985 Dec;5(12):3610-6 PMID: 3915782
  24. Amino acid sequences common to rapidly degraded proteins: the PEST hypothesis.
    Science. 1986 Oct 17;234(4774):364-8 PMID: 2876518
  25. Crystal structure at 2.4 angstroms resolution of the complex of transducin betagamma and its regulator, phosducin.
    Cell. 1996 Nov 1;87(3):577-88 PMID: 8898209
  26. How proteolysis drives the cell cycle.
    Science. 1996 Dec 6;274(5293):1652-9 PMID: 8939846
  27. Cdc53p acts in concert with Cdc4p and Cdc34p to control the G1-to-S-phase transition and identifies a conserved family of proteins.
    Mol Cell Biol. 1996 Dec;16(12):6634-43 PMID: 8943317
  28. Structure, function, and expression of SEL-1, a negative regulator of LIN-12 and GLP-1 in C. elegans.
    Development. 1997 Feb;124(3):637-44 PMID: 9043078
  29. Notch activity influences the alphabeta versus gammadelta T cell lineage decision.
    Cell. 1997 Mar 21;88(6):833-43 PMID: 9118226
  30. F-box proteins are receptors that recruit phosphorylated substrates to the SCF ubiquitin-ligase complex.
    Cell. 1997 Oct 17;91(2):209-19 PMID: 9346238
  31. A complex of Cdc4p, Skp1p, and Cdc53p/cullin catalyzes ubiquitination of the phosphorylated CDK inhibitor Sic1p.
    Cell. 1997 Oct 17;91(2):221-30 PMID: 9346239
  32. The retinoblastoma protein associates with the protein phosphatase type 1 catalytic subunit.
    Genes Dev. 1993 Apr;7(4):555-69 PMID: 8384581
  33. A trans-spliced leader sequence on actin mRNA in C. elegans.
    Cell. 1987 Jun 19;49(6):753-61 PMID: 3581169
  34. The yeast cell cycle gene CDC34 encodes a ubiquitin-conjugating enzyme.
    Science. 1988 Sep 9;241(4871):1331-5 PMID: 2842867
  35. The basal component of the nematode dense-body is vinculin.
    J Biol Chem. 1989 Jun 15;264(17):10177-85 PMID: 2498337
  36. Cell autonomy of lin-12 function in a cell fate decision in C. elegans.
    Cell. 1989 Jun 30;57(7):1237-45 PMID: 2736627
  37. A novel genetic system to detect protein-protein interactions.
    Nature. 1989 Jul 20;340(6230):245-6 PMID: 2547163
  38. glp-1 and lin-12, genes implicated in distinct cell-cell interactions in C. elegans, encode similar transmembrane proteins.
    Cell. 1989 Aug 11;58(3):553-63 PMID: 2758466
  39. Notch is required for successive cell decisions in the developing Drosophila retina.
    Genes Dev. 1989 Aug;3(8):1099-112 PMID: 2792755
  40. Improved retroviral vectors for gene transfer and expression.
    Biotechniques. 1989 Oct;7(9):980-2, 984-6, 989-90 PMID: 2631796
  41. Analysis of gain-of-function mutations of the lin-12 gene of Caenorhabditis elegans.
    Nature. 1990 Jul 12;346(6280):197-9 PMID: 2164160
  42. Basic local alignment search tool.
    J Mol Biol. 1990 Oct 5;215(3):403-10 PMID: 2231712
  43. The production of single-stranded DNA suitable for sequencing using the polymerase chain reaction.
    Biotechniques. 1991 Jan;10(1):24-6 PMID: 2003916
  44. The choice of cell fate in the epidermis of Drosophila.
    Cell. 1991 Mar 22;64(6):1083-92 PMID: 2004417
  45. TAN-1, the human homolog of the Drosophila notch gene, is broken by chromosomal translocations in T lymphoblastic neoplasms.
    Cell. 1991 Aug 23;66(4):649-61 PMID: 1831692
  46. A conserved family of nuclear phosphoproteins localized to sites of polymerase II transcription.
    J Cell Biol. 1991 Nov;115(3):587-96 PMID: 1717489
  47. Efficient gene transfer in C.elegans: extrachromosomal maintenance and integration of transforming sequences.
    EMBO J. 1991 Dec;10(12):3959-70 PMID: 1935914
  48. Analysis of dominant-negative mutations of the Caenorhabditis elegans let-60 ras gene.
    Genes Dev. 1991 Dec;5(12A):2188-98 PMID: 1748278
  49. Mouse mammary tumor gene int-3: a member of the notch gene family transforms mammary epithelial cells.
    J Virol. 1992 Apr;66(4):2594-9 PMID: 1312643
  50. Efficient production of single-stranded DNA as long as 2 kb for sequencing of PCR-amplified DNA.
    Biotechniques. 1992 Feb;12(2):164, 166, 168-71 PMID: 1616702
  51. At least four regulatory genes control sulphur metabolite repression in Aspergillus nidulans.
    Mol Gen Genet. 1993 Apr;238(1-2):185-92 PMID: 8479426
  52. Intrinsic activity of the Lin-12 and Notch intracellular domains in vivo.
    Cell. 1993 Jul 30;74(2):331-45 PMID: 8343960
  53. Production of high-titer helper-free retroviruses by transient transfection.
    Proc Natl Acad Sci U S A. 1993 Sep 15;90(18):8392-6 PMID: 7690960
  54. Suppressors of a lin-12 hypomorph define genes that interact with both lin-12 and glp-1 in Caenorhabditis elegans.
    Genetics. 1993 Nov;135(3):765-83 PMID: 8293978
  55. The ubiquitin-mediated proteolytic pathway: mechanisms of recognition of the proteolytic substrate and involvement in the degradation of native cellular proteins.
    FASEB J. 1994 Feb;8(2):182-91 PMID: 8119489
  56. The postembryonic cell lineages of the hermaphrodite and male gonads in Caenorhabditis elegans.
    Dev Biol. 1979 Jun;70(2):396-417 PMID: 478167
Article Info
Journal
Genes & development
Abbr.
Genes Dev
ISSN
0890-9369
DOI
10.1101/gad.11.23.3182
Published
1997-12-01
Pages
3182-93
Language
English
Region
United States
NLM ID
8711660
PMCID
PMC316751
Subset
IM
Grants
NIGMS NIH HHS · GM37602 · United States
Databases
GENBANK
AF020788
External Links
PubMed source DOI: 10.1101/gad.11.23.3182 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2