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PMID: 9420229 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Infectious clones and vectors derived from adeno-associated virus (AAV) serotypes other than AAV type 2.

Journal of virology ·Vol. 72 ·No. 1 ·1998-01-00 ·Pages 309-19

Rutledge EA, Halbert CL, Russell DW

Abstract

Adeno-associated viruses (AAVs) are single-stranded dependent parvoviruses being developed as transducing vectors. Although at least five serotypes exist (AAV types 1 to 5 [AAV1 to -5]), only AAV2, AAV3, and AAV4 have been sequenced, and the vectors in use were almost all derived from AAV2. Here we report the cloning and sequencing of a second AAV3 genome and a new AAV serotype designated AAV6 that is related to AAV1. AAV2, AAV3, and AAV6 were 82% identical at the nucleotide sequence level, and AAV4 was 75 to 78% identical to these AAVs. Significant sequence variation was noted in portions of the capsid proteins that presumably are responsible for serotype-specific functions. Vectors produced from AAV3 and AAV6 differed from AAV2 vectors in host range and serologic reactivity. The AAV3 and AAV6 vector serotypes were able to transduce cells in the presence of serum from animals previously exposed to AAV2 vectors. Our results suggest that vectors based on alternative AAV serotypes will have advantages over existing AAV2 vectors, including the transduction of different cell types, and resistance to neutralizing antibodies against AAV2. This could be especially important for gene therapy, as significant immunity against AAV2 exists in human populations and many protocols will likely require multiple vector doses.

MeSH Terms
Amino Acid Sequence Animals Antibodies, Viral Base Sequence Cell Line Cloning, Molecular DNA Primers/genetics DNA, Viral/chemistry,genetics Dependovirus/classification,genetics,immunology Genetic Variation Genetic Vectors Genome, Viral Humans Molecular Sequence Data Nucleic Acid Conformation Repetitive Sequences, Nucleic Acid Sequence Homology, Amino Acid Sequence Homology, Nucleic Acid Serotyping Transduction, Genetic
Chemicals
Antibodies, Viral DNA Primers DNA, Viral
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Rutledge E A
Division of Hematology and Markey Molecular Medicine Center, University of Washington, Seattle 98195, USA.
Halbert C L
Russell D W
References (59)
59 references, click to expand
  1. Adeno-associated virus vector integration junctions.
    J Virol. 1997 Nov;71(11):8429-36 PMID: 9343199
  2. A maltose-binding protein/adeno-associated virus Rep68 fusion protein has DNA-RNA helicase and ATPase activities.
    J Virol. 1995 Jun;69(6):3542-8 PMID: 7538173
  3. Efficient long-term gene transfer into muscle tissue of immunocompetent mice by adeno-associated virus vector.
    J Virol. 1996 Nov;70(11):8098-108 PMID: 8892935
  4. Nucleotide sequencing and generation of an infectious clone of adeno-associated virus 3.
    Virology. 1996 Jul 1;221(1):208-17 PMID: 8661429
  5. A minute circular DNA from Escherichia coli 15.
    Proc Natl Acad Sci U S A. 1968 Jul;60(3):992-9 PMID: 4875809
  6. A recombinant plasmid from which an infectious adeno-associated virus genome can be excised in vitro and its use to study viral replication.
    J Virol. 1987 Oct;61(10):3096-101 PMID: 3041032
  7. Epidemiology of adenovirus-associated virus infection in a nursery population.
    Am J Epidemiol. 1968 Nov;88(3):368-78 PMID: 4301609
  8. Characterization of a newly derived human sarcoma cell line (HT-1080).
    Cancer. 1974 Apr;33(4):1027-33 PMID: 4132053
  9. Conserved arrangement of nested genes at the Drosophila Gart locus.
    Genetics. 1987 Dec;117(4):711-25 PMID: 3123310
  10. Cloning of infectious adeno-associated virus genomes in bacterial plasmids.
    Gene. 1983 Jul;23(1):65-73 PMID: 6352411
  11. Sequence requirements for stable binding and function of Rep68 on the adeno-associated virus type 2 inverted terminal repeats.
    J Virol. 1994 Nov;68(11):7448-57 PMID: 7933128
  12. Nucleotide sequence and organization of the adeno-associated virus 2 genome.
    J Virol. 1983 Feb;45(2):555-64 PMID: 6300419
  13. Recombinant adeno-associated virus-mediated high-efficiency, transient expression of the murine cationic amino acid transporter (ecotropic retroviral receptor) permits stable transduction of human HeLa cells by ecotropic retroviral vectors.
    J Virol. 1996 Oct;70(10):6759-66 PMID: 8794313
  14. Mutational analysis of adeno-associated virus Rep protein-mediated inhibition of heterologous and homologous promoters.
    J Virol. 1995 Sep;69(9):5485-96 PMID: 7636994
  15. Adeno-associated virus vectors transduce primary cells much less efficiently than immortalized cells.
    J Virol. 1995 Mar;69(3):1473-9 PMID: 7853479
  16. Rapid and efficient site-specific mutagenesis without phenotypic selection.
    Methods Enzymol. 1987;154:367-82 PMID: 3323813
  17. Characterization of the DNA of a defective human parvovirus isolated from a genital site.
    Virology. 1984 Apr 15;134(1):52-63 PMID: 6324476
  18. The recombination signals for adeno-associated virus site-specific integration.
    Proc Natl Acad Sci U S A. 1996 Jul 23;93(15):7966-72 PMID: 8755586
  19. Polyoma transformation of hamster cell clones--an investigation of genetic factors affecting cell competence.
    Virology. 1962 Feb;16:147-51 PMID: 14468055
  20. A system for shotgun DNA sequencing.
    Nucleic Acids Res. 1981 Jan 24;9(2):309-21 PMID: 6259625
  21. An Escherichia coli recBCsbcBrecF host permits the deletion-resistant propagation of plasmid clones containing the 5'-terminal palindrome of minute virus of mice.
    Gene. 1985;35(1-2):179-85 PMID: 3896934
  22. Seroepidemiological and ecological studies of the adenovirus-associated satellite viruses.
    Infect Immun. 1970 Dec;2(6):716-22 PMID: 16557905
  23. Recombinant adeno-associated virus mediates a high level of gene transfer but less efficient integration in the K562 human hematopoietic cell line.
    J Virol. 1997 Mar;71(3):1776-83 PMID: 9032306
  24. Interactions between the termini of adeno-associated virus DNA.
    J Mol Biol. 1989 Mar 5;206(1):91-100 PMID: 2539485
  25. Studies of small DNA viruses found in various adenovirus preparations: physical, biological, and immunological characteristics.
    Proc Natl Acad Sci U S A. 1966 Jun;55(6):1467-74 PMID: 5227666
  26. Physical assay and growth cycle studies of a defective adeno-satellite virus.
    J Virol. 1967 Feb;1(1):171-80 PMID: 4990036
  27. A novel 165-base-pair terminal repeat sequence is the sole cis requirement for the adeno-associated virus life cycle.
    J Virol. 1997 Feb;71(2):941-8 PMID: 8995611
  28. Mutations in the carboxy terminus of adeno-associated virus 2 capsid proteins affect viral infectivity: lack of an RGD integrin-binding motif.
    J Gen Virol. 1994 Dec;75 ( Pt 12):3385-92 PMID: 7996133
  29. Human chronic myelogenous leukemia cell-line with positive Philadelphia chromosome.
    Blood. 1975 Mar;45(3):321-34 PMID: 163658
  30. Features of the adeno-associated virus origin involved in substrate recognition by the viral Rep protein.
    J Virol. 1993 Oct;67(10):6096-104 PMID: 8396670
  31. High-level globin gene expression mediated by a recombinant adeno-associated virus genome that contains the 3' gamma globin gene regulatory element and integrates as tandem copies in erythroid cells.
    Blood. 1997 Mar 15;89(6):2167-75 PMID: 9058741
  32. Transduction by adeno-associated virus vectors in the rabbit airway: efficiency, persistence, and readministration.
    J Virol. 1997 Aug;71(8):5932-41 PMID: 9223483
  33. Murine retroviruses use at least six different receptors for entry into Mus dunni cells.
    J Virol. 1997 Jun;71(6):4531-5 PMID: 9151846
  34. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  35. Effects of gamma irradiation on the transduction of dividing and nondividing cells in brain and muscle of rats by adeno-associated virus vectors.
    Hum Gene Ther. 1996 May 1;7(7):841-50 PMID: 8860836
  36. Analysis of mutation in human cells by using an Epstein-Barr virus shuttle system.
    Mol Cell Biol. 1987 Jan;7(1):379-87 PMID: 3031469
  37. Differential expression in human cells from the p6 promoter of human parvovirus B19 following plasmid transfection and recombinant adeno-associated virus 2 (AAV) infection: human megakaryocytic leukaemia cells are non-permissive for AAV infection.
    J Gen Virol. 1996 Jun;77 ( Pt 6):1111-22 PMID: 8683195
  38. Cloning of adeno-associated virus type 4 (AAV4) and generation of recombinant AAV4 particles.
    J Virol. 1997 Sep;71(9):6823-33 PMID: 9261407
  39. Second-strand synthesis is a rate-limiting step for efficient transduction by recombinant adeno-associated virus vectors.
    J Virol. 1996 May;70(5):3227-34 PMID: 8627803
  40. A cystic fibrosis bronchial epithelial cell line: immortalization by adeno-12-SV40 infection.
    Am J Respir Cell Mol Biol. 1991 Apr;4(4):313-9 PMID: 1849726
  41. The adeno-associated virus type 2 p40 promoter requires a proximal Sp1 interaction and a p19 CArG-like element to facilitate Rep transactivation.
    J Virol. 1997 Jun;71(6):4300-9 PMID: 9151818
  42. Use of adeno-associated virus as a general transduction vector for mammalian cells.
    Curr Top Microbiol Immunol. 1992;158:97-129 PMID: 1316261
  43. Organization of the adeno-associated virus (AAV) capsid gene: mapping of a minor spliced mRNA coding for virus capsid protein 1.
    Virology. 1988 Nov;167(1):176-84 PMID: 2847413
  44. Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid.
    J Bacteriol. 1978 Jun;134(3):1141-56 PMID: 149110
  45. Structure and nucleotide sequence of the terminal regions of adeno-associated virus DNA.
    Virology. 1977 May 15;78(2):475-7 PMID: 194395
  46. Sequence requirements for binding of Rep68 to the adeno-associated virus terminal repeats.
    J Virol. 1996 Mar;70(3):1542-53 PMID: 8627673
  47. In vivo model of adeno-associated virus vector persistence and rescue.
    J Virol. 1996 May;70(5):3235-41 PMID: 8627804
  48. Transduction with recombinant adeno-associated virus for gene therapy is limited by leading-strand synthesis.
    J Virol. 1996 Jan;70(1):520-32 PMID: 8523565
  49. The cellular transcription factor SP1 and an unknown cellular protein are required to mediate Rep protein activation of the adeno-associated virus p19 promoter.
    J Virol. 1997 Mar;71(3):1747-56 PMID: 9032303
  50. Molecular cloning of adeno-associated virus variant genomes and generation of infectious virus by recombination in mammalian cells.
    J Biol Chem. 1984 Apr 10;259(7):4661-6 PMID: 6323482
  51. Serologic evidence for human infection with adenovirus-associated viruses.
    J Natl Cancer Inst. 1968 Feb;40(2):319-27 PMID: 4295610
  52. The P-loop--a common motif in ATP- and GTP-binding proteins.
    Trends Biochem Sci. 1990 Nov;15(11):430-4 PMID: 2126155
  53. Adeno-associated virus type 2 binds to a 150-kilodalton cell membrane glycoprotein.
    Virology. 1996 Mar 1;217(1):124-30 PMID: 8599196
  54. A recombinant retrovirus encoding alkaline phosphatase confirms clonal boundary assignment in lineage analysis of murine retina.
    Proc Natl Acad Sci U S A. 1992 Jan 15;89(2):693-7 PMID: 1731342
  55. Characteristics of a human cell line transformed by DNA from human adenovirus type 5.
    J Gen Virol. 1977 Jul;36(1):59-74 PMID: 886304
  56. Adeno-associated virus vectors preferentially transduce cells in S phase.
    Proc Natl Acad Sci U S A. 1994 Sep 13;91(19):8915-9 PMID: 8090744
  57. Helper-free stocks of recombinant adeno-associated viruses: normal integration does not require viral gene expression.
    J Virol. 1989 Sep;63(9):3822-8 PMID: 2547998
  58. Analysis of proteins, helper dependence, and seroepidemiology of a new human parvovirus.
    Virology. 1984 Apr 15;134(1):64-71 PMID: 6200995
  59. ADENOVIRUS-ASSOCIATED DEFECTIVE VIRUS PARTICLES.
    Science. 1965 Aug 13;149(3685):754-6 PMID: 14325163
Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.72.1.309-319.1998
Published
1998-01-00
Pages
309-19
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC109378
Subset
IM
Databases
GENBANK
AF028704, AF028705
External Links
PubMed source DOI: 10.1128/JVI.72.1.309-319.1998 PMC Full Text View journal details
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