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PMID: 9435237 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Receptors for anti-müllerian hormone on Leydig cells are responsible for its effects on steroidogenesis and cell differentiation.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 95 ·No. 2 ·1998-01-20 ·Pages 594-9

Racine C, Rey R, Forest MG, Louis F, Ferré A, Huhtaniemi I, Josso N, di Clemente N

Abstract

Strong overexpression of anti-Müllerian hormone (AMH) in transgenic mice leads to incomplete fetal virilization and decreased serum testosterone in the adult. Conversely, AMH-deficient mice exhibit Leydig cell hyperplasia. To probe the mechanism of action of AMH on Leydig cell steroidogenesis, we have studied the expression of mRNA for steroidogenic proteins in vivo and in vitro and performed a morphometric analysis of testicular tissue in mice overexpressing the hormone. We show that overexpression of AMH in male transgenic mice blocks the differentiation of Leydig cell precursors. Expression of steroidogenic protein mRNAs, mainly cytochrome P450 17 alpha-hydroxylase/C17-20 lyase (P450c17), is decreased in transgenic mice overexpressing AMH and in AMH-treated purified Leydig cells. In contrast, transgenic mice in whom the AMH locus has been disrupted show increase expression of P450c17. In vitro, but not in vivo, AMH also decreases the expression of the luteinizing hormone receptor. The effect of AMH is explained by the presence of its receptor on Leydig cells. Our results provide insight into the action of AMH as a negative modulator of Leydig cell differentiation and function.

MeSH Terms
Animals Anti-Mullerian Hormone Cell Differentiation/physiology Cells, Cultured Gene Transfer Techniques Glycoproteins Growth Inhibitors/physiology Leydig Cells/cytology,physiology Male Mice Mice, Transgenic Receptors, Peptide/physiology Receptors, Transforming Growth Factor beta Signal Transduction Steroids/metabolism Testicular Hormones/physiology
Chemicals
Glycoproteins Growth Inhibitors Receptors, Peptide Receptors, Transforming Growth Factor beta Steroids Testicular Hormones anti-Mullerian hormone receptor Anti-Mullerian Hormone
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Racine C
Institut National de la Santé et de la Recherche Médicale, Ecole Normale Supérieure, Département de Biologie, Montrouge, France.
Rey R
Forest M G
Louis F
Ferré A
Huhtaniemi I
Josso N
di Clemente N
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.95.2.594
Published
1998-01-20
Pages
594-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC18465
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.95.2.594 PMC Full Text View journal details
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