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PMID: 9436903 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Conservation of structure and subunit interactions in yeast homologues of splicing factor 3b (SF3b) subunits.

RNA (New York, N.Y.) ·Vol. 4 ·No. 1 ·1998-01-00 ·Pages 1-10

Igel H, Wells S, Perriman R, Ares M

Abstract

Human SAP 49, a subunit of the multimeric splicing factor 3b (SF3b), contains two RNA recognition motifs (RRMs) and binds another SF3b subunit called SAP 145, whose yeast homologue is CUS1. Here we show that the predicted yeast open reading frame YOR319w (HSH49) encodes an essential yeast splicing factor. Using bacterially expressed proteins, we find that yeast HSH49 binds CUS1. Mutations that alter putative RNA-binding residues of either HSH49 RRM are lethal in vivo, but do not prevent binding to CUS1 in vitro, suggesting that the predicted RNA-binding surfaces of HSH49 are not required for interaction with CUS1. In vivo interaction tests show that HSH49 and CUS1 associate primarily through the N-terminal RRM of HSH49. Recombinant HSH49 protein has a general RNA-binding activity that does not require CUS1. The parallels in structure and interaction between two SF3b subunits from yeast implies that the mechanism of SF3b action is highly conserved.

MeSH Terms
Amino Acid Sequence Binding Sites Conserved Sequence Fungal Proteins/chemistry,genetics,metabolism Molecular Sequence Data RNA Splicing RNA Splicing Factors RNA, Fungal/metabolism RNA-Binding Proteins/chemistry,genetics,metabolism Recombinant Fusion Proteins/genetics,metabolism Ribonucleoprotein, U2 Small Nuclear Saccharomyces cerevisiae/genetics Saccharomyces cerevisiae Proteins Sequence Homology, Amino Acid
Chemicals
CUS1 protein, S cerevisiae Fungal Proteins HSH49 protein, S cerevisiae RNA Splicing Factors RNA, Fungal RNA-Binding Proteins Recombinant Fusion Proteins Ribonucleoprotein, U2 Small Nuclear SF3B2 protein, human SF3B4 protein, human Saccharomyces cerevisiae Proteins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Igel H
Center for the Molecular Biology of RNA, Biology Department, University of California at Santa Cruz, 95064, USA.
Wells S
Perriman R
Ares M
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Article Info
Journal
RNA (New York, N.Y.)
Abbr.
RNA
ISSN
1355-8382
Published
1998-01-00
Pages
1-10
Language
English
Region
United States
NLM ID
9509184
PMCID
PMC1369591
Subset
IM
Grants
NIGMS NIH HHS · GM40478 · United States
External Links
PubMed source PMC Full Text View journal details
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