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PMID: 9449708 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A gene encoding antigenic peptides of human squamous cell carcinoma recognized by cytotoxic T lymphocytes.

The Journal of experimental medicine ·Vol. 187 ·No. 3 ·1998-02-02 ·Pages 277-88

Shichijo S, Nakao M, Imai Y, Takasu H, Kawamoto M, Niiya F, Yang D, Toh Y, Yamana H, Itoh K

Abstract

Except for melanomas, tumor antigens recognized by cytotoxic T lymphocytes (CTLs) are yet unidentified. We have identified a gene encoding antigenic peptides of human squamous cell carcinomas (SCCs) recognized by human histocompatibility leukocyte antigens (HLA)- A2601-restricted CTLs. This gene showed no similarity to known sequences, and encoded two (125- and 43-kilodalton [kD]) proteins. The 125-kD protein with the leucine zipper motif was expressed in the nucleus of the majority of proliferating cells tested, including normal and malignant cells. The 43-kD protein was expressed in the cytosol of most SCCs from various organs and half of lung adenocarcinomas, but was not expressed in other cancers nor in a panel of normal tissues. The three nonapeptides shared by the two proteins were recognized by the KE4 CTLs, and one of the peptides induced in vitro from peripheral blood mononuclear cells (PBMCs) the CTLs restricted to the autologous tumor cells. The 43-kD protein and this nonapeptide (KGSGKMKTE) may be useful for the specific immunotherapy of HLA-A2601(+) epithelial cancer patients.

MeSH Terms
Amino Acid Sequence Antigens, Neoplasm/analysis,chemistry,immunology Base Sequence Blotting, Western Carcinoma, Squamous Cell/chemistry,immunology Cloning, Molecular Gene Expression Regulation, Neoplastic/genetics HLA Antigens/immunology Humans Immunotherapy Interferon-gamma/metabolism Leucine Zippers/genetics Molecular Sequence Data Neoplasm Proteins/chemistry Peptide Fragments/chemistry,genetics,pharmacology Peptides/chemistry,immunology,therapeutic use RNA, Messenger/analysis Ribonucleoproteins, Small Nuclear Sequence Analysis, DNA Sequence Deletion/genetics T-Lymphocytes, Cytotoxic/immunology,metabolism
Chemicals
Antigens, Neoplasm HLA Antigens Neoplasm Proteins Peptide Fragments Peptides RNA, Messenger Ribonucleoproteins, Small Nuclear SART1 protein, human Interferon-gamma
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Shichijo S
Department of Immunology, Kurume University School of Medicine, Kurume, Japan.
Nakao M
Imai Y
Takasu H
Kawamoto M
Niiya F
Yang D
Toh Y
Yamana H
Itoh K
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.187.3.277
Published
1998-02-02
Pages
277-88
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2212124
Subset
IM
Databases
GENBANK
AB006198
External Links
PubMed source DOI: 10.1084/jem.187.3.277 PMC Full Text View journal details
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