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PMID: 9456309 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Chromosome association of minichromosome maintenance proteins in Drosophila endoreplication cycles.

The Journal of cell biology ·Vol. 140 ·No. 3 ·1998-02-09 ·Pages 451-60

Su TT, O'Farrell PH

Abstract

Minichromosome maintenance (MCM) proteins are essential eukaryotic DNA replication factors. The binding of MCMs to chromatin oscillates in conjunction with progress through the mitotic cell cycle. This oscillation is thought to play an important role in coupling DNA replication to mitosis and limiting chromosome duplication to once per cell cycle. The coupling of DNA replication to mitosis is absent in Drosophila endoreplication cycles (endocycles), during which discrete rounds of chromosome duplication occur without intervening mitoses. We examined the behavior of MCM proteins in endoreplicating larval salivary glands, to determine whether oscillation of MCM-chromosome localization occurs in conjunction with passage through an endocycle S phase. We found that MCMs in polytene nuclei exist in two states: associated with or dissociated from chromosomes. We demonstrate that cyclin E can drive chromosome association of DmMCM2 and that DNA synthesis erases this association. We conclude that mitosis is not required for oscillations in chromosome binding of MCMs and propose that cycles of MCM-chromosome association normally occur in endocycles. These results are discussed in a model in which the cycle of MCM-chromosome associations is uncoupled from mitosis because of the distinctive program of cyclin expression in endocycles.

MeSH Terms
Animals Aphidicolin/pharmacology Chromosomes/metabolism Cyclin E/biosynthesis DNA/biosynthesis DNA Replication Drosophila/cytology,embryology,metabolism Insect Proteins/analysis,metabolism Larva/metabolism Mitosis S Phase Salivary Glands/cytology,metabolism
Chemicals
Cyclin E Insect Proteins Aphidicolin DNA
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Su T T
Department of Biochemistry and Biophysics, University of California San Francisco, San Francisco, California 94143-0448, USA.
O'Farrell P H
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.140.3.451
Published
1998-02-09
Pages
451-60
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2140170
Subset
IM
Grants
NIGMS NIH HHS · R01 GM037193 · United States
NIGMS NIH HHS · GM15032 · United States
NIGMS NIH HHS · R01-GM57193 · United States
External Links
PubMed source DOI: 10.1083/jcb.140.3.451 PMC Full Text View journal details
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