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PMID: 9462212 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Cytokine mRNA expression in intestinal tissue of interleukin-2 deficient mice with bowel inflammation.

Gut ·Vol. 41 ·No. 6 ·1997-12-00 ·Pages 793-800

Autenrieth IB, Bucheler N, Bohn E, Heinze G, Horak I

Abstract

Mice deficient in interleukin-2 (IL-2) develop inflammatory bowel disease resembling ulcerative colitis in humans. Recent studies provided evidence that alpha beta T cells, particularly CD4 T cells, rather than B cells, are involved in the pathogenesis of bowel inflammation of IL-2 deficient mice. To analyse the pattern of expression of cytokine mRNA in intestinal tissue of normal and IL-2 deficient mice. Expression of beta-actin, IL-1 alpha, IL-1 beta, IL-6, IL-10, tumour necrosis factor alpha (TNF-alpha), interferon gamma (IFN-gamma) and transforming growth factor beta 1 (TGF-beta 1) mRNA was analysed in colon and small intestinal tissue of both IL-2 deficient (IL-2-/-) mice and normal (wild type) litter mates (IL-2+/+) at different ages by using qualitative, as well as semiquantitative, competitive reverse transcription polymerase chain reaction (RT-PCR). Results were correlated with the phase of progression of the disease, as determined by histology. IL-2-/- mice had expressed low levels of IL-1 alpha, IL-1 beta, IL-6, TNF-alpha, and IFN-gamma mRNA in the colon by 1.5 weeks of age. In advance of the development of histologically and clinically detectable bowel inflammation, expression of IL-1 alpha, IL-1 beta, IL-6, TNF-alpha, IFN-gamma, and IL-10, but not TGF-beta 1, mRNA increased in the colon of IL-2 deficient mice. In contrast, IL-2+/+ mice expressed TGF-beta 1 mRNA in colon tissue at 13 and 23 weeks of age, but not IL-1 alpha, IL-1 beta, IL-6, TNF-alpha, IL-10, or IFN-gamma mRNA. Levels of expression of cytokine mRNA in tissue from the small intestine were comparable in IL-2-/- and IL-2+/+ mice. Bowel inflammation in IL-2 deficient mice is preceded by an increase in IL-1 alpha, IL-1 beta, TNF-alpha, and IFN-gamma mRNA expression in colon tissue. Low levels of TGF-beta 1, but high levels of IL-1 alpha, IL-1 beta, IL-6, TNF-alpha, IFN-gamma, and IL-10 mRNA expression correlate with the manifestation of severe colitis, and suggest that T cells and macrophages are involved in bowel inflammation of IL-2 deficient mice.

MeSH Terms
Actins/genetics Animals Colon/immunology Cytokines/genetics,metabolism Gene Expression Inflammatory Bowel Diseases/immunology,metabolism Interferon-gamma/genetics Interleukin-1/genetics Interleukin-10/genetics Interleukin-2/deficiency Interleukin-6/genetics Intestinal Mucosa/metabolism Intestine, Small/immunology Intestines/immunology Mice Mice, Inbred C57BL Mice, Mutant Strains Polymerase Chain Reaction RNA, Messenger/analysis,metabolism Transforming Growth Factor beta/genetics Tumor Necrosis Factor-alpha/genetics
Chemicals
Actins Cytokines Interleukin-1 Interleukin-2 Interleukin-6 RNA, Messenger Transforming Growth Factor beta Tumor Necrosis Factor-alpha Interleukin-10 Interferon-gamma
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Autenrieth I B
Max v. Pettenkofer Institut für Hygiene und Medizinische Mikrobiologie, Ludwig Maximilians Universität, München, Germany.
Bucheler N
Bohn E
Heinze G
Horak I
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Article Info
Journal
Gut
Abbr.
Gut
ISSN
0017-5749
DOI
10.1136/gut.41.6.793
Published
1997-12-00
Pages
793-800
Language
English
Region
England
NLM ID
2985108R
PMCID
PMC1891612
Subset
IM
External Links
PubMed source DOI: 10.1136/gut.41.6.793 PMC Full Text View journal details
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