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PMID: 9477573 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Involvement of maize Dof zinc finger proteins in tissue-specific and light-regulated gene expression.

The Plant cell ·Vol. 10 ·No. 1 ·1998-01-00 ·Pages 75-89

Yanagisawa S, Sheen J

Abstract

Dof is a novel family of plant proteins that share a unique and highly conserved DNA binding domain with one C2-C2 zinc finger motif. Although multiple Dof proteins associated with diverse gene promoters have recently been identified in a variety of plants, their physiological functions and regulation remain elusive. In maize, Dof1 (MNB1a) is constitutively expressed in leaves, stems, and roots, whereas the closely related Dof2 is expressed mainly in stems and roots. Here, by using a maize leaf protoplast transient assay, we show that Dof1 is a transcriptional activator, whereas Dof2 can act as a transcriptional repressor. Thus, differential expression of Dof1 and Dof2 may permit leaf-specific gene expression. Interestingly, in vivo analyses showed that although DNA binding activity of Dof1 is regulated by light-dependent development, its transactivation activity and nuclear localization are not. Moreover, in vivo transcription and in vitro electrophoretic mobility shift assays revealed that Dof1 can interact specifically with the maize C4 phosphoenolpyruvate carboxylase gene promoter and enhance its promoter activity, which displays a light-regulated expression pattern matching Dof1 activity. We propose that the evolutionarily conserved Dof proteins can function as transcriptional activators or repressors of tissue-specific and light-regulated gene expression in plants.

MeSH Terms
Amino Acid Sequence Cell Compartmentation Cell Nucleus/chemistry Cells, Cultured DNA-Binding Proteins/metabolism Darkness Gene Expression Regulation, Plant Green Fluorescent Proteins Light Luminescent Proteins/genetics,isolation & purification Models, Genetic Molecular Sequence Data Plant Leaves/cytology,genetics,radiation effects Plant Proteins/metabolism Promoter Regions, Genetic Protein Binding Protoplasts Recombinant Fusion Proteins/isolation & purification Repressor Proteins/metabolism Transcription Factors/metabolism Transcription, Genetic Zea mays/genetics,radiation effects Zinc Fingers
Chemicals
DNA-Binding Proteins Luminescent Proteins MNB1A protein, Zea mays Plant Proteins Recombinant Fusion Proteins Repressor Proteins Transcription Factors Green Fluorescent Proteins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Yanagisawa S
Department of Life Sciences (Chemistry), Graduate School of Arts and Sciences, University of Tokyo, Japan. [email protected]
Sheen J
References (66)
66 references, click to expand
  1. A CACGTG motif of the Antirrhinum majus chalcone synthase promoter is recognized by an evolutionarily conserved nuclear protein.
    Proc Natl Acad Sci U S A. 1989 Sep;86(18):6930-4 PMID: 2780550
  2. Maize phosphoenolpyruvate carboxylase involved in C4 photosynthesis: nucleotide sequence analysis of the 5' flanking region of the gene.
    J Biochem. 1989 Dec;106(6):982-7 PMID: 2628434
  3. Metabolic repression of transcription in higher plants.
    Plant Cell. 1990 Oct;2(10):1027-38 PMID: 2136626
  4. An evolutionarily conserved protein binding sequence upstream of a plant light-regulated gene.
    Proc Natl Acad Sci U S A. 1988 Oct;85(19):7089-93 PMID: 2902624
  5. The promoter of a H2O2-inducible, Arabidopsis glutathione S-transferase gene contains closely linked OBF- and OBP1-binding sites.
    Plant J. 1996 Dec;10(6):955-66 PMID: 9011080
  6. Nuclear targeting in plants.
    Plant Physiol. 1992 Dec;100(4):1627-32 PMID: 16653177
  7. Mediator of transcriptional regulation.
    Trends Biochem Sci. 1996 Sep;21(9):335-7 PMID: 8870496
  8. A novel multigene family that the gene for a maize DNA-binding protein, MNB1a belongs to: isolation of genomic clones from this family and some aspects of its molecular evolution.
    Biochem Mol Biol Int. 1996 Apr;38(4):665-73 PMID: 8728095
  9. The G-box: a ubiquitous regulatory DNA element in plants bound by the GBF family of bZIP proteins.
    Trends Biochem Sci. 1995 Dec;20(12):506-10 PMID: 8571452
  10. A tobacco bZip transcription activator (TAF-1) binds to a G-box-like motif conserved in plant genes.
    EMBO J. 1991 Jul;10(7):1793-802 PMID: 2050116
  11. Sugar sensing in higher plants.
    Plant Cell. 1994 Nov;6(11):1665-79 PMID: 7827498
  12. The phosphoenolpyruvate carboxylase gene family of Sorghum: promoter structures, amino acid sequences and expression of genes.
    Gene. 1991 Mar 1;99(1):87-94 PMID: 2022326
  13. A tobacco DNA binding protein that interacts with a light-responsive box II element.
    Plant Cell. 1992 Jul;4(7):831-8 PMID: 1392597
  14. A rolB regulatory factor belongs to a new class of single zinc finger plant proteins.
    Plant J. 1996 Aug;10(2):215-23 PMID: 8771779
  15. Maize polyubiquitin genes: structure, thermal perturbation of expression and transcript splicing, and promoter activity following transfer to protoplasts by electroporation.
    Plant Mol Biol. 1992 Feb;18(4):675-89 PMID: 1313711
  16. The Cauliflower Mosaic Virus 35S Promoter: Combinatorial Regulation of Transcription in Plants.
    Science. 1990 Nov 16;250(4983):959-66 PMID: 17746920
  17. Deletion analysis of GAL4 defines two transcriptional activating segments.
    Cell. 1987 Mar 13;48(5):847-53 PMID: 3028647
  18. Characterization of a gene encoding a DNA binding protein with specificity for a light-responsive element.
    Plant Cell. 1992 Jul;4(7):839-49 PMID: 1392598
  19. Sequence-specific interactions of a pea nuclear factor with light-responsive elements upstream of the rbcS-3A gene.
    EMBO J. 1987 Sep;6(9):2543-9 PMID: 3678200
  20. Engineered GFP as a vital reporter in plants.
    Curr Biol. 1996 Mar 1;6(3):325-30 PMID: 8805250
  21. LIGHT CONTROL OF SEEDLING DEVELOPMENT.
    Annu Rev Plant Physiol Plant Mol Biol. 1996 Jun;47:215-243 PMID: 15012288
  22. A plant DNA-binding protein increases the number of active preinitiation complexes in a human in vitro transcription system.
    Genes Dev. 1990 Nov;4(11):1899-909 PMID: 2276624
  23. Cell-specific accumulation of maize phosphoenolpyruvate carboxylase is correlated with demethylation at a specific site greater than 3 kb upstream of the gene.
    Mol Gen Genet. 1991 Jan;225(1):49-55 PMID: 2000091
  24. Two 10-bp regions are critical for phytochrome regulation of a Lemna gibba Lhcb gene promoter.
    Plant Cell. 1994 Aug;6(8):1123-34 PMID: 7919982
  25. Dof DNA-binding domains of plant transcription factors contribute to multiple protein-protein interactions.
    Eur J Biochem. 1997 Dec 1;250(2):403-10 PMID: 9428691
  26. GUS fusions: beta-glucuronidase as a sensitive and versatile gene fusion marker in higher plants.
    EMBO J. 1987 Dec 20;6(13):3901-7 PMID: 3327686
  27. Green-fluorescent protein as a new vital marker in plant cells.
    Plant J. 1995 Nov;8(5):777-84 PMID: 8528289
  28. A novel DNA-binding domain that may form a single zinc finger motif.
    Nucleic Acids Res. 1995 Sep 11;23(17):3403-10 PMID: 7567449
  29. Out of darkness: mutants reveal pathways controlling light-regulated development in plants.
    Trends Genet. 1993 May;9(5):167-72 PMID: 8337753
  30. Sp1-mediated transcriptional activation is repressed by Sp3.
    EMBO J. 1994 Aug 15;13(16):3843-51 PMID: 8070411
  31. A flower-specific Myb protein activates transcription of phenylpropanoid biosynthetic genes.
    EMBO J. 1994 Jan 1;13(1):128-37 PMID: 8306956
  32. Interactions between distinct types of DNA binding proteins enhance binding to ocs element promoter sequences.
    Plant Cell. 1995 Dec;7(12):2241-52 PMID: 8718629
  33. The rice bZIP transcriptional activator RITA-1 is highly expressed during seed development.
    Plant Cell. 1994 Sep;6(9):1277-87 PMID: 7919992
  34. A maize zinc-finger protein binds the prolamin box in zein gene promoters and interacts with the basic leucine zipper transcriptional activator Opaque2.
    Proc Natl Acad Sci U S A. 1997 Jul 8;94(14):7685-90 PMID: 9207153
  35. Photoregulated gene expression may involve ubiquitous DNA binding proteins.
    EMBO J. 1990 Nov;9(11):3415-27 PMID: 2209551
  36. Dual DNA binding specificity of a petal epidermis-specific MYB transcription factor (MYB.Ph3) from Petunia hybrida.
    EMBO J. 1995 Apr 18;14(8):1773-84 PMID: 7737128
  37. Plant bZIP protein DNA binding specificity.
    J Mol Biol. 1993 Apr 20;230(4):1131-44 PMID: 8487298
  38. DNA binding site preferences and transcriptional activation properties of the Arabidopsis transcription factor GBF1.
    EMBO J. 1992 Apr;11(4):1275-89 PMID: 1563344
  39. A trans-acting factor that binds to a GT-motif in a phytochrome gene promoter.
    Science. 1990 Dec 7;250(4986):1397-9 PMID: 2255908
  40. Ca2+-dependent protein kinases and stress signal transduction in plants.
    Science. 1996 Dec 13;274(5294):1900-2 PMID: 8943201
  41. Maize C4 photosynthesis involves differential regulation of phosphoenolpyruvate carboxylase genes.
    Plant J. 1992 Mar;2(2):221-32 PMID: 1302051
  42. Opaque-2 is a transcriptional activator that recognizes a specific target site in 22-kD zein genes.
    Plant Cell. 1992 Jun;4(6):689-700 PMID: 1392590
  43. Phytochromes: photosensory perception and signal transduction.
    Science. 1995 May 5;268(5211):675-80 PMID: 7732376
  44. A metal-dependent DNA-binding protein interacts with a constitutive element of a light-responsive promoter.
    Plant Cell. 1990 Sep;2(9):857-66 PMID: 2152132
  45. Differential expression of C4 pathway genes in mesophyll and bundle sheath cells of greening maize leaves.
    J Biol Chem. 1987 Aug 25;262(24):11726-30 PMID: 2442151
  46. From seed germination to flowering, light controls plant development via the pigment phytochrome.
    Proc Natl Acad Sci U S A. 1996 Oct 29;93(22):12066-71 PMID: 8901532
  47. The maize regulatory locus Opaque-2 encodes a DNA-binding protein which activates the transcription of the b-32 gene.
    EMBO J. 1991 Mar;10(3):617-24 PMID: 2001677
  48. Protein phosphatase activity is required for light-inducible gene expression in maize.
    EMBO J. 1993 Sep;12(9):3497-505 PMID: 8253076
  49. Molecular cloning of two DNA-binding proteins of maize that are structurally different but interact with the same sequence motif.
    J Biol Chem. 1993 Jul 25;268(21):16028-36 PMID: 8340424
  50. A DNA binding activity for one of two closely defined phytochrome regulatory elements in an Lhcb promoter is more abundant in etiolated than in green plants.
    Plant Cell. 1996 Jan;8(1):31-41 PMID: 8597658
  51. Zinc finger-mediated protein interactions modulate Ikaros activity, a molecular control of lymphocyte development.
    EMBO J. 1996 Oct 1;15(19):5358-69 PMID: 8895580
  52. Molecular mechanisms underlying the differential expression of maize pyruvate, orthophosphate dikinase genes.
    Plant Cell. 1991 Mar;3(3):225-45 PMID: 1668653
  53. A transcriptional activation domain of ATMYB2, a drought-inducible Arabidopsis Myb-related protein.
    Plant J. 1996 Dec;10(6):1145-8 PMID: 9011094
  54. Plant transcription factors: present knowledge and future challenges.
    Trends Genet. 1992 Jan;8(1):22-7 PMID: 1369731
  55. GT-1 binding site confers light responsive expression in transgenic tobacco.
    Science. 1990 Apr 27;248(4954):471-4 PMID: 2330508
  56. Binding sites for two novel phosphoproteins, 3AF5 and 3AF3, are required for rbcS-3A expression.
    Plant Cell. 1992 Apr;4(4):473-83 PMID: 1498605
  57. Yeast activators stimulate plant gene expression.
    Nature. 1988 Aug 18;334(6183):631-3 PMID: 3165494
  58. Phytochrome phototransduction pathways.
    Annu Rev Genet. 1994;28:325-49 PMID: 7893130
  59. Maize rbcS promoter activity depends on sequence elements not found in dicot rbcS promoters.
    Plant Cell. 1991 Sep;3(9):997-1012 PMID: 1822995
  60. A novel DNA binding protein with homology to Myb oncoproteins containing only one repeat can function as a transcriptional activator.
    EMBO J. 1994 Nov 15;13(22):5383-92 PMID: 7957104
  61. MYB transcription factors in plants.
    Trends Genet. 1997 Feb;13(2):67-73 PMID: 9055608
  62. GT-2: in vivo transcriptional activation activity and definition of novel twin DNA binding domains with reciprocal target sequence selectivity.
    Plant Cell. 1996 Jun;8(6):1041-59 PMID: 8672890
  63. GT-2: a transcription factor with twin autonomous DNA-binding domains of closely related but different target sequence specificity.
    EMBO J. 1992 Nov;11(11):4131-44 PMID: 1396594
  64. Heterodimerization between light-regulated and ubiquitously expressed Arabidopsis GBF bZIP proteins.
    EMBO J. 1992 Apr;11(4):1261-73 PMID: 1373374
  65. Evolution and expression of C4 photosynthesis genes.
    Plant Physiol. 1996 Aug;111(4):949-57 PMID: 8756491
  66. Light-inducible and constitutively expressed DNA-binding proteins recognizing a plant promoter element with functional relevance in light responsiveness.
    EMBO J. 1991 Jul;10(7):1777-86 PMID: 2050115
Article Info
Journal
The Plant cell
Abbr.
Plant Cell
ISSN
1040-4651
DOI
10.1105/tpc.10.1.75
Email
[email protected]
Published
1998-01-00
Pages
75-89
Language
English
Region
England
NLM ID
9208688
PMCID
PMC143930
Subset
IM
External Links
PubMed source DOI: 10.1105/tpc.10.1.75 PMC Full Text View journal details
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