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PMID: 9491998 Published · ppublish English Journal Article

Massive expansion of antigen-specific CD8+ T cells during an acute virus infection.

Immunity ·Vol. 8 ·No. 2 ·1998-02-00 ·Pages 167-75

Butz EA, Bevan MJ

Abstract

During LCMV infection, CD8+ T cells expand greatly. Bystander activation has been thought to play a role because few cells score as LCMV specific in limiting dilution analysis. In contrast, we find that at least a quarter of the CD8+ cells secrete IFNgamma specifically in response to LCMV peptides at the peak of the response. Moreover, by analyzing the expansion of adoptively transferred LCMV-specific, TCR-transgenic CD8+ T cells in congenic hosts, we have determined that most of the CD8+ cell expansion is virus specific. Analysis of the effect of the monospecific TCR-transgenic T cells on the host response to three LCMV epitopes suggests that CTL precursors compete for sites on the APC in an epitope-specific fashion and that this competition determines the specificity of the response.

MeSH Terms
Acute Disease Adoptive Transfer Animals Antigen-Presenting Cells/immunology Antigens, Viral/immunology CD8-Positive T-Lymphocytes/immunology Epitopes Histocompatibility Antigens Class I/immunology Interferon-gamma/metabolism Lymphocyte Activation Lymphocytic Choriomeningitis/immunology Mice Mice, Inbred C57BL Mice, Transgenic Models, Immunological Peptides/immunology Receptors, Antigen, T-Cell/genetics Spleen/cytology,immunology T-Lymphocyte Subsets/immunology T-Lymphocytes, Cytotoxic Transplantation Chimera
Chemicals
Antigens, Viral Epitopes Histocompatibility Antigens Class I Peptides Receptors, Antigen, T-Cell Interferon-gamma
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Butz E A
Howard Hughes Medical Institute, Department of Immunology, University of Washington, Seattle 98195, USA.
Bevan M J
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Article Info
Journal
Immunity
Abbr.
Immunity
ISSN
1074-7613
DOI
10.1016/s1074-7613(00)80469-0
Published
1998-02-00
Pages
167-75
Language
English
Region
United States
NLM ID
9432918
PMCID
PMC2776648
Subset
IM
Grants
Howard Hughes Medical Institute · United States
NIAID NIH HHS · R01 AI019335 · United States
NIAID NIH HHS · R01 AI019335-19 · United States
External Links
PubMed source DOI: 10.1016/s1074-7613(00)80469-0 PMC Full Text View journal details
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