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PMID: 9502413 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Expression of heme oxygenase-1 in atherosclerotic lesions.

The American journal of pathology ·Vol. 152 ·No. 3 ·1998-03-00 ·Pages 711-20

Wang LJ, Lee TS, Lee FY, Pai RC, Chau LY

Abstract

Heme oxygenase-1 (HO-1) is a heme-degradation enzyme induced under various oxidative stress conditions. To elucidate the potential involvement of HO-1 in atherogenesis, the expression of this enzyme in atherosclerotic lesions of apolipoprotein E-deficient mice and humans were examined. Both immunostaining and in situ hybridization clearly demonstrated that the expression of HO-1 was prominent in endothelium and foam cells/macrophages of thickened intima in lesions from both humans and experimental animals. The expression of this enzyme was also detected in medial smooth muscle cells of advanced lesions. The induction of HO-1 mRNA was observed in murine peritoneal macrophages after treatment with oxidized low density lipoprotein (LDL) but not with native LDL in a dose-dependent manner. Time course study demonstrated that the induction was prominent at 3 hours, reached a maximal induction at 6 hours, and remained evident up to 24 hours after oxidized LDL treatment. The degree of induction was in concordant with the extent of oxidation in the LDL preparation. Lysophosphatidylcholine, one of the major components present in oxidized LDL, was ineffective to induce the gene expression, suggesting that other lipophilic substances derived from LDL oxidation are responsible for the induction of HO-1. These results clearly demonstrate that HO-1 is one of the stress proteins expressed in atherosclerotic lesions.

MeSH Terms
Aged Aged, 80 and over Animals Aorta/enzymology,pathology Arteriosclerosis/enzymology,pathology Cells, Cultured Dose-Response Relationship, Drug Heme Oxygenase (Decyclizing)/genetics,metabolism Heme Oxygenase-1 Humans Immunohistochemistry In Situ Hybridization Lipoproteins, LDL/pharmacology Macrophages, Peritoneal/drug effects,enzymology Male Membrane Proteins Mice Mice, Inbred C57BL Mice, Mutant Strains Middle Aged Muscle, Smooth, Vascular/drug effects,enzymology RNA, Messenger/metabolism
Chemicals
Lipoproteins, LDL Membrane Proteins RNA, Messenger HMOX1 protein, human Heme Oxygenase (Decyclizing) Heme Oxygenase-1 Hmox1 protein, mouse
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Wang L J
Division of Cardiovascular Research, Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan, Republic of China.
Lee T S
Lee F Y
Pai R C
Chau L Y
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Article Info
Journal
The American journal of pathology
Abbr.
Am J Pathol
ISSN
0002-9440
Published
1998-03-00
Pages
711-20
Language
English
Region
United States
NLM ID
0370502
PMCID
PMC1858397
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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