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PMID: 9526507 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Identification of UV/blue light-response elements in the Arabidopsis thaliana chalcone synthase promoter using a homologous protoplast transient expression system.

Plant molecular biology ·Vol. 36 ·No. 5 ·1998-03-00 ·Pages 741-54

Hartmann U, Valentine WJ, Christie JM, Hays J, Jenkins GI, Weisshaar B

Abstract

To identify DNA sequences of the Arabidopsis thaliana chalcone synthase gene (CHS) concerned with induction by UV-B and UV-A/blue light, AtCHS promoter constructions were assayed by transient expression in protoplasts prepared from two different lines of cultured A. thaliana cells. The protoplasts responded similarly to A. thaliana leaf tissue in light-dependent CHS transcript accumulation. The reporter enzyme beta-glucuronidase (GUS) was used to monitor light-responsive promoter activity. A 1972 bp promoter conferred UV-B and UV-A/blue light induction of GUS activity. Deletion to 164 bp resulted in reduced promoter strength but retention of responsiveness to UV-B and UV-A/blue light. Further deletion abolished transcriptional activity. The 164 bp promoter contains sequences closely resembling LRUPcCHS, (light-responsive unit of the Petroselinum crispum CHS promoter). This A. thaliana CHS promoter region, designated LRUAtCHS, was sufficient to confer UV-B and UV-A/blue light responsiveness to a heterologous core promoter. Mutation of sequences in LRUAtCHS corresponding to the ACGT element and the MYB recognition element of LRUPcCHS resulted in inactivation of the 164 bp and 335 bp promoter deletions. However, the mutant 668 bp promoter retained residual UV-B and UV-A/blue light-induced expression, indicating the presence of additional functional sequences upstream of -335. Mutation of a single G-box-like sequence around -442 had no effect on light responsiveness, indicating that it does not function in light regulation of this promoter. Since no difference in responsiveness to UV-B and UV-A/blue light was observed with any promoter variant, we conclude that the two phototransduction pathways regulate transcription factors which interact with common promoter elements. The results from-our analysis of a A. thaliana light-responsive promoter will facilitate the study of light-dependent gene regulation by genetic means in Arabidopsis thaliana.

MeSH Terms
Acyltransferases/genetics Arabidopsis/enzymology,genetics,radiation effects Base Sequence DNA, Plant/genetics Gene Expression Regulation, Plant/radiation effects Genes, Plant/radiation effects Genetic Variation Light Molecular Sequence Data Mutagenesis Promoter Regions, Genetic/radiation effects Protoplasts/radiation effects Sequence Deletion Ultraviolet Rays
Chemicals
DNA, Plant Acyltransferases flavanone synthetase
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Hartmann U
Max-Planck-Institut für Züchtungsforschung, Abteilung Biochemie, Köln, Germany.
Valentine W J
Christie J M
Hays J
Jenkins G I
Weisshaar B
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Article Info
Journal
Plant molecular biology
Abbr.
Plant Mol Biol
ISSN
0167-4412
DOI
10.1023/a:1005921914384
Published
1998-03-00
Pages
741-54
Language
English
Region
Netherlands
NLM ID
9106343
Subset
IM
Databases
GENBANK
AF012810
External Links
PubMed source DOI: 10.1023/a:1005921914384 View journal details
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