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PMID: 9557731 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Cryoelectron microscopic examination of human immunodeficiency virus type 1 virions with mutations in the cyclophilin A binding loop.

Journal of virology ·Vol. 72 ·No. 5 ·1998-05-00 ·Pages 4403-7

Kong LB, An D, Ackerson B, Canon J, Rey O, Chen IS, Krogstad P, Stewart PL

Abstract

The human immunodeficiency virus type 1 capsid protein contains a conserved P217X4PX2PX5P231 motif. Mutation at Pro-222 decreases virion incorporation of cyclophilin A, while mutation at Pro-231 abolishes infectivity. Although viral RNA incorporation and protease cleavage of the Gag precursor were not affected by these mutations, cryoelectron microscopy revealed a loss of virion maturation in P231A particles.

MeSH Terms
Binding Sites Cell Line Endopeptidases/metabolism Gene Products, gag/metabolism HIV-1/genetics,metabolism,physiology,ultrastructure Humans Microscopy, Electron Mutagenesis, Site-Directed Peptidylprolyl Isomerase/metabolism Virion/ultrastructure Virus Replication
Chemicals
Gene Products, gag Endopeptidases Peptidylprolyl Isomerase
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Kong L B
Department of Molecular and Medical Pharmacology and Crump Institute for Biological Imaging, UCLA School of Medicine, Los Angeles, California 90095, USA.
An D
Ackerson B
Canon J
Rey O
Chen I S
Krogstad P
Stewart P L
References (44)
44 references, click to expand
  1. Further evidence of icosahedral symmetry in human and simian immunodeficiency virus.
    AIDS Res Hum Retroviruses. 1993 Oct;9(10):929-38 PMID: 8280478
  2. QVIEW: software for rapid selection of particles from digital electron micrographs.
    J Struct Biol. 1998 Sep;123(1):17-21 PMID: 9774540
  3. Specific incorporation of cyclophilin A into HIV-1 virions.
    Nature. 1994 Nov 24;372(6504):359-62 PMID: 7969494
  4. Functional association of cyclophilin A with HIV-1 virions.
    Nature. 1994 Nov 24;372(6504):363-5 PMID: 7969495
  5. Linker insertion mutations in the human immunodeficiency virus type 1 gag gene: effects on virion particle assembly, release, and infectivity.
    J Virol. 1995 Feb;69(2):642-50 PMID: 7815527
  6. Vpr-induced cell cycle arrest is conserved among primate lentiviruses.
    J Virol. 1996 Apr;70(4):2516-24 PMID: 8642681
  7. Cyclophilin A is required for an early step in the life cycle of human immunodeficiency virus type 1 before the initiation of reverse transcription.
    J Virol. 1996 Jun;70(6):3551-60 PMID: 8648689
  8. HIV-1 Gag protein associates with F-actin present in microfilaments.
    Virology. 1996 Jun 15;220(2):530-4 PMID: 8661406
  9. Structure of the amino-terminal core domain of the HIV-1 capsid protein.
    Science. 1996 Jul 12;273(5272):231-5 PMID: 8662505
  10. Economical apparatus for safe, accurate recovery of biohazardous or radioactive gradient fractions.
    Biotechniques. 1996 May;20(5):878-9 PMID: 8723935
  11. Crystal structure of dimeric HIV-1 capsid protein.
    Nat Struct Biol. 1996 Sep;3(9):763-70 PMID: 8784350
  12. Comparative morphology and structural classification of retroviruses.
    Curr Top Microbiol Immunol. 1996;214:1-24 PMID: 8791723
  13. Quantitative analysis of the endogenous reverse transcriptase reactions of HIV type 1 variants with decreased susceptibility to azidothymidine and nevirapine.
    AIDS Res Hum Retroviruses. 1996 Jul 20;12(11):977-83 PMID: 8827213
  14. Ultrastructure and morphogenesis of human immunodeficiency virus.
    J Electron Microsc (Tokyo). 1996 Aug;45(4):247-57 PMID: 8888583
  15. Cryo-electron microscopy of viruses.
    Nature. 1984 Mar 1-7;308(5954):32-6 PMID: 6322001
  16. Production of acquired immunodeficiency syndrome-associated retrovirus in human and nonhuman cells transfected with an infectious molecular clone.
    J Virol. 1986 Aug;59(2):284-91 PMID: 3016298
  17. The gag gene products of human immunodeficiency virus type 1: alignment within the gag open reading frame, identification of posttranslational modifications, and evidence for alternative gag precursors.
    J Virol. 1988 Nov;62(11):3993-4002 PMID: 3262776
  18. HIV-1 entry into quiescent primary lymphocytes: molecular analysis reveals a labile, latent viral structure.
    Cell. 1990 Apr 20;61(2):213-22 PMID: 2331748
  19. Sequence-specific binding of DNA by the Moloney murine leukemia virus integrase protein.
    J Virol. 1990 Jun;64(6):2796-801 PMID: 2186176
  20. Effect of mutations affecting the p6 gag protein on human immunodeficiency virus particle release.
    Proc Natl Acad Sci U S A. 1991 Apr 15;88(8):3195-9 PMID: 2014240
  21. Maturation of human immunodeficiency virus particles assembled from the gag precursor protein requires in situ processing by gag-pol protease.
    AIDS Res Hum Retroviruses. 1991 May;7(5):475-83 PMID: 1873082
  22. Assembly and morphology of HIV: potential effect of structure on viral function.
    AIDS. 1991 Jun;5(6):617-37 PMID: 1652977
  23. Form, function, and use of retroviral gag proteins.
    AIDS. 1991 Jun;5(6):639-54 PMID: 1883539
  24. Spatial visualization of the maturing HIV-1 core and its linkage to the envelope.
    AIDS Res Hum Retroviruses. 1992 Jan;8(1):1-7 PMID: 1736937
  25. Incompletely reverse-transcribed human immunodeficiency virus type 1 genomes in quiescent cells can function as intermediates in the retroviral life cycle.
    J Virol. 1992 Mar;66(3):1717-25 PMID: 1371173
  26. Gag proteins of the highly replicative MN strain of human immunodeficiency virus type 1: posttranslational modifications, proteolytic processings, and complete amino acid sequences.
    J Virol. 1992 Apr;66(4):1856-65 PMID: 1548743
  27. Structure of a human rhinovirus complexed with its receptor molecule.
    Proc Natl Acad Sci U S A. 1993 Jan 15;90(2):507-11 PMID: 8093643
  28. Partial inhibition of the human immunodeficiency virus type 1 protease results in aberrant virus assembly and the formation of noninfectious particles.
    J Virol. 1993 Jul;67(7):4050-5 PMID: 8510215
  29. Assembly, processing, and infectivity of human immunodeficiency virus type 1 gag mutants.
    J Virol. 1993 Jul;67(7):4264-73 PMID: 7685414
  30. Human immunodeficiency virus type 1 Gag protein binds to cyclophilins A and B.
    Cell. 1993 Jun 18;73(6):1067-78 PMID: 8513493
  31. Fullerene-like organization of HIV gag-protein shell in virus-like particles produced by recombinant baculovirus.
    Virology. 1994 Jan;198(1):288-96 PMID: 8259664
  32. The role of Gag in human immunodeficiency virus type 1 virion morphogenesis and early steps of the viral life cycle.
    J Virol. 1996 Dec;70(12):8645-52 PMID: 8970990
  33. Absconding with the chaperone: essential cyclophilin-Gag interaction in HIV-1 virions.
    Cell. 1996 Dec 27;87(7):1157-9 PMID: 8980222
  34. Crystal structure of human cyclophilin A bound to the amino-terminal domain of HIV-1 capsid.
    Cell. 1996 Dec 27;87(7):1285-94 PMID: 8980234
  35. High-efficiency transduction of human lymphoid progenitor cells and expression in differentiated T cells.
    J Virol. 1997 Feb;71(2):1397-404 PMID: 8995665
  36. The hydrophobic pocket of cyclophilin is the binding site for the human immunodeficiency virus type 1 Gag polyprotein.
    J Virol. 1997 Mar;71(3):2107-13 PMID: 9032343
  37. Determination of the fold of the core protein of hepatitis B virus by electron cryomicroscopy.
    Nature. 1997 Mar 6;386(6620):88-91 PMID: 9052786
  38. Visualization of a 4-helix bundle in the hepatitis B virus capsid by cryo-electron microscopy.
    Nature. 1997 Mar 6;386(6620):91-4 PMID: 9052787
  39. Cryo-EM visualization of an exposed RGD epitope on adenovirus that escapes antibody neutralization.
    EMBO J. 1997 Mar 17;16(6):1189-98 PMID: 9135136
  40. Molecular recognition in the HIV-1 capsid/cyclophilin A complex.
    J Mol Biol. 1997 Jun 27;269(5):780-95 PMID: 9223641
  41. Cyclophilin A-induced alterations of human immunodeficiency virus type 1 CA protein in vitro.
    J Virol. 1997 Sep;71(9):6921-7 PMID: 9261419
  42. Cryo-electron microscopy reveals ordered domains in the immature HIV-1 particle.
    Curr Biol. 1997 Oct 1;7(10):729-38 PMID: 9368755
  43. Cells with high cyclophilin A content support replication of human immunodeficiency virus type 1 Gag mutants with decreased ability to incorporate cyclophilin A.
    J Virol. 1998 Jan;72(1):303-8 PMID: 9420228
  44. Functional domains of the capsid protein of human immunodeficiency virus type 1.
    J Virol. 1994 Dec;68(12):8180-7 PMID: 7966609
Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.72.5.4403-4407.1998
Published
1998-05-00
Pages
4403-7
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC109671
Subset
IM
Grants
NIAID NIH HHS · AI28697 · United States
NIAID NIH HHS · P30 AI028697 · United States
NIAID NIH HHS · AI07388-07 · United States
NIAID NIH HHS · AI01144 · United States
NIAID NIH HHS · T32 AI007388 · United States
External Links
PubMed source DOI: 10.1128/JVI.72.5.4403-4407.1998 PMC Full Text View journal details
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