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PMID: 8648689 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Cyclophilin A is required for an early step in the life cycle of human immunodeficiency virus type 1 before the initiation of reverse transcription.

Journal of virology ·Vol. 70 ·No. 6 ·1996-06-00 ·Pages 3551-60

Braaten D, Franke EK, Luban J

Abstract

Cyclophilin A (CyPA) is incorporated into human immunodeficiency virus type 1 (HIV-1) virions via contact with the Gag polyprotein. Genetic or pharmacologic disruption of CyPA incorporation causes a quantitative reduction in virion infectivity with no discernible effects on virion assembly or on endogenous reverse transcriptase activity. Instead, the reduction of virion-associated CyPA is accompanied by a parallel, quantitative decrease in the initiation of viral DNA synthesis after infection of T cells. The infectivity of CyPA-deficient virions is not restored by pseudotyping with Env of amphotropic murine leukemia virus, demonstrating that CyPA is not required for the HIV-1-Env-CD4 interaction. These results indicate that CyPA is required for an early step in the HIV-1 life cycle following receptor binding and membrane fusion but preceding reverse transcription. CyPA is the first cellular protein other than the cell surface receptor shown to be required for an early event in the life cycle of a retrovirus.

MeSH Terms
Amino Acid Isomerases/physiology Base Sequence Carrier Proteins/physiology DNA, Viral/biosynthesis Gene Products, gag/metabolism HIV-1/physiology Humans Membrane Fusion Molecular Sequence Data Peptidylprolyl Isomerase Transcription, Genetic Virion/physiology Virus Assembly Virus Replication
Chemicals
Carrier Proteins DNA, Viral Gene Products, gag Amino Acid Isomerases Peptidylprolyl Isomerase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Braaten D
Department of Microbiology, College of Physicians and Surgeons, Columbia University, New York 10032, USA.
Franke E K
Luban J
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.70.6.3551-3560.1996
Published
1996-06-00
Pages
3551-60
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC190230
Subset
IM
Grants
NIAID NIH HHS · R01 AI036199 · United States
NIAID NIH HHS · AI36199 · United States
External Links
PubMed source DOI: 10.1128/JVI.70.6.3551-3560.1996 PMC Full Text View journal details
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