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PMID: 1326652 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Fv-1 restriction and its effects on murine leukemia virus integration in vivo and in vitro.

Journal of virology ·Vol. 66 ·No. 10 ·1992-10-00 ·Pages 5959-66

Pryciak PM, Varmus HE

Abstract

We have investigated the mechanisms by which alleles at the mouse Fv-1 locus restrict replication of murine leukemia viruses. Inhibition of productive infection is closely paralleled by reduced accumulation of integrated proviral DNA as well as by reduced levels of linear viral DNA in a cytoplasmic fraction. Nevertheless, viral DNA is present at nearly normal levels in a nuclear fraction, and total amounts of viral DNA are only mildly affected in restrictive infections, suggesting a block in integration to account for reduced levels of proviral DNA. However, integrase (IN)-dependent trimming of 3' ends of viral DNA occurs normally in vivo during restrictive infections, demonstrating that not all IN-mediated events are prevented in vivo. Furthermore, viral integration complexes present in nuclear extracts of infected restrictive cells are fully competent to integrate their DNA into a heterologous target in vitro. Thus, the Fv-1-dependent activity that restricts integration in vivo may be lost in vitro; alternatively, Fv-1 restriction may prevent a step required for integration in vivo that is bypassed in vitro.

Related Genes
Fv-1
MeSH Terms
3T3 Cells Animals DNA, Viral/analysis Genes, Viral Leukemia Virus, Murine/genetics,physiology Mice Mice, Inbred BALB C Plasmids Virus Integration
Chemicals
DNA, Viral
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Pryciak P M
Department of Biochemistry and Biophysics, University of California, San Francisco 94143-0502.
Varmus H E
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.66.10.5959-5966.1992
Published
1992-10-00
Pages
5959-66
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC241473
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.66.10.5959-5966.1992 PMC Full Text View journal details
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