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PMID: 206900 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Transfection of Fv-1 permissive and restrictive mouse cells with integrated DNA of murine leukemia viruses.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 75 ·No. 3 ·1978-03-00 ·Pages 1451-5

Hsu IC, Yang WK, Tennant RW, Brown A

Abstract

Whole-cell DNA preparations isolated from SC-1 cells chronically infected with N- or B-tropic murine leukemia viruses (MuLV) were tested for infectious activity in an Fv-1n (NIH-3T3) and two Fv-Ib (C57BL/6 and SV-A31) cell cultures. Efficiency of transfection for all DNAs was better in the NIH-3T3 cells than in C57BL/6 or SV-A31 cells; and an [N-tropic MuLV]SC-1 cell DNA preparation was slightly more infectious than a [B-tropic MuLV]SC-1 cell DNA preparation in all three cell cultures, regardless of their Fv-1 genotypes. Progeny viruses from the transfection showed N- or B-tropism corresponding to that of the parent viruses produced by the infected SC-1 cells that were used for the DNA preparation. DNA dose-response studies in NIH-3T3 cells revealed a one-hit mechanism for both the [B-tropic MuLV]SC-1 cell DNA and the [N-tropic MuLV]SC-1 cell DNA preparation. These results demonstrate that, in contrast to virion infection, transfection of N- and B-tropic MuLV with DNA preparations from chronically infected cells is not affected by the Fv-1 gene.

MeSH Terms
Cell Line DNA/genetics Genes Leukemia Virus, Murine/genetics,growth & development Transfection
Chemicals
DNA
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Hsu I C
Yang W K
Tennant R W
Brown A
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32 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.75.3.1451
Published
1978-03-00
Pages
1451-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC411490
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.75.3.1451 PMC Full Text View journal details
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