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PMID: 6310140 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Reversal of Fv-1 host range by in vitro restriction endonuclease fragment exchange between molecular clones of N-tropic and B-tropic murine leukemia virus genomes.

Journal of virology ·Vol. 48 ·No. 1 ·1983-10-00 ·Pages 110-9

Boone LR, Myer FE, Yang DM, Ou CY, Koh CK, Roberson LE, Tennant RW, Yang WK

Abstract

We molecularly cloned unintegrated viral DNA of the BALB/c endogenous N-tropic and B-tropic murine leukemia retroviruses and in vitro passaged N-tropic Gross (passage A) murine leukemia retroviruses. Recombinant genomes were constructed in vitro by exchanging homologous restriction enzyme fragments from N- or B-tropic parents and subsequent recloning. Infectious virus was recovered after transfection of these recombinant genomes into NIH-3T3 cells and cocultivation with the Fv-1 nonrestrictive SC-1 cells. XC plaque assays of recombinant virus progeny on Fv-ln and Fv-lb cells indicated that the Fv-l host range was determined by sequences located between the BamHI site in the p30 region of the gag gene (1.6 kilobase pairs from the left end of the map) and the HindIII site located in the pol gene (2.9 kilobase pairs from the left end of the map).

MeSH Terms

AKR murine leukemia virus/genetics,physiology Animals Cell Line Cloning, Molecular DNA Restriction Enzymes DNA, Recombinant Genes, Viral Leukemia Virus, Murine/genetics,physiology Mice Transfection

Chemicals

DNA, Recombinant DNA Restriction Enzymes

Authors & Affiliations

8 authors, click to expand affiliations / ORCID

Boone L R

Myer F E

Yang D M

Ou C Y

Koh C K

Roberson L E

Tennant R W

Yang W K

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33 references, click to expand

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Article Info

Journal

Journal of virology

Abbr.

J Virol

ISSN

0022-538X

DOI

10.1128/JVI.48.1.110-119.1983

Published

1983-10-00

Pages

110-9

Language

English

Region

United States

NLM ID

0113724

PMCID

PMC255327

Subset

Grants

NCI NIH HHS · CA 30308 · United States

External Links

PubMed source DOI: 10.1128/JVI.48.1.110-119.1983 PMC Full Text View journal details

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Reversal of Fv-1 host range by in vitro restriction endonuclease fragment exchange between molecular clones of N-tropic and B-tropic murine leukemia virus genomes.

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