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PMID: 2173775 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Efficient autointegration of avian retrovirus DNA in vitro.

Journal of virology ·Vol. 64 ·No. 12 ·1990-12-00 ·Pages 5958-65

Lee YM, Coffin JM

Abstract

We have developed a cell-free system for an avian retrovirus that promotes autointegration, one-long-terminal-repeat (LTR) circle formation, and correct integration into exogenous target DNA. In this system, autointegration and one-LTR circle formation occurred far more frequently than integration into exogenous target DNA. Autointegration had the same characteristics of normal integration into target DNA except in its selection of target. Highly efficient autointegration as well as one-LTR circle formation in vitro suggest that there may be a mechanism to prevent these processes in vivo.

MeSH Terms
Animals Avian Sarcoma Viruses/genetics Bacteriophage lambda/genetics Base Sequence Cell Line Cell Transformation, Neoplastic DNA, Circular/genetics,isolation & purification DNA, Viral/biosynthesis,genetics,isolation & purification Kinetics Lysogeny Molecular Sequence Data Molecular Weight Nucleic Acid Hybridization Quail Repetitive Sequences, Nucleic Acid
Chemicals
DNA, Circular DNA, Viral
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Lee Y M
Department of Molecular Biology and Microbiology, Tufts University School of Medicine, Boston, Massachusetts 02111.
Coffin J M
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33 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.64.12.5958-5965.1990
Published
1990-12-00
Pages
5958-65
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC248769
Subset
IM
Grants
NCI NIH HHS · CA17659 · United States
NCI NIH HHS · CA44385 · United States
External Links
PubMed source DOI: 10.1128/JVI.64.12.5958-5965.1990 PMC Full Text View journal details
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