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PMID: 9576758 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

The agent of Human Granulocytic Ehrlichiosis resides in an endosomal compartment.

The Journal of clinical investigation ·Vol. 101 ·No. 9 ·1998-05-01 ·Pages 1932-41

Webster P, IJdo JW, Chicoine LM, Fikrig E

Abstract

The composition of cytoplasmic vacuoles containing the agent of Human Granulocytic Ehrlichiosis (HGE) was studied to investigate how this pathogen exists within infected host cells. Electron microscopy demonstrated that the HGE organism resides in a membrane-bound compartment within HL-60 cells: early forms of the HGE agent have a round reticular appearance while later structures are small and dense. Vacuoles containing HGE bacteria incorporated endocytosed colloidal gold particles, suggesting that they are part of the endocytic pathway. Antibodies directed to the mannose-6-phosphate receptor labeled vacuole membranes. Antibodies to the transferrin receptor and to the lysosomal membrane glycoprotein LAMP 1 did not. Moreover, 3-(2,4-dinitroanilino)-3'-amino-N-methyldipropylamine, which normally accumulates in compartments with low pH, was not present inside these vacuoles. These results suggest that vacuoles containing the agent of HGE fail to mature into phagolysosomes. We conclude that the agent of HGE appears to enter and modify part of the endocytic pathway.

MeSH Terms
Cell Compartmentation Ehrlichia/growth & development Endocytosis Endosomes/microbiology,ultrastructure Humans Neutrophils/microbiology,ultrastructure
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Webster P
Department of Cell Biology, Yale University School of Medicine, New Haven, Connecticut 06520-8031, USA.
IJdo J W
Chicoine L M
Fikrig E
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Article Info
Journal
The Journal of clinical investigation
Abbr.
J Clin Invest
ISSN
0021-9738
DOI
10.1172/JCI1544
Published
1998-05-01
Pages
1932-41
Language
English
Region
United States
NLM ID
7802877
PMCID
PMC508780
Subset
IM
Grants
NIAID NIH HHS · AI-41440 · United States
External Links
PubMed source DOI: 10.1172/JCI1544 PMC Full Text View journal details
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