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PMID: 9649503 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Identification of Xenopus SMC protein complexes required for sister chromatid cohesion.

Genes & development ·Vol. 12 ·No. 13 ·1998-07-01 ·Pages 1986-97

Losada A, Hirano M, Hirano T

Abstract

The structural maintenance of chromosomes (SMC) family is a growing family of chromosomal ATPases. The founding class of SMC protein complexes, condensins, plays a central role in mitotic chromosome condensation. We report here a new class of SMC protein complexes containing XSMC1 and XSMC3, Xenopus homologs of yeast Smc1p and Smc3p, respectively. The protein complexes (termed cohesins) exist as two major forms with sedimentation coefficients of 9S and 14S. 9S cohesin is a heterodimer of XSMC1 and XSMC3, whereas 14S cohesin contains three additional subunits. One of them has been identified as a Xenopus homolog of the Schizosaccharomyces pombe Rad21p implicated in DNA repair and the Saccharomyces cerevisiae Scc1p/Mcd1p implicated in sister chromatid cohesion. 14S cohesin binds to interphase chromatin independently of DNA replication and dissociates from it at the onset of mitosis. Immunodepletion of cohesins during interphase causes defects in sister chromatid cohesion in subsequent mitosis, whereas condensation is unaffected. These results suggest that proper assembly of mitotic chromosomes is regulated by two distinct classes of SMC protein complexes, cohesins and condensins.

MeSH Terms
Adenosine Triphosphatases/genetics,physiology Amino Acid Sequence Animals Cell Cycle Proteins/genetics,isolation & purification,physiology Chromatids/genetics,physiology Chromatin/metabolism Chromosomal Proteins, Non-Histone Cloning, Molecular DNA Replication/genetics DNA-Binding Proteins/genetics,isolation & purification,physiology Fungal Proteins/genetics Interphase/genetics Macromolecular Substances Mitosis/genetics Molecular Sequence Data Molecular Weight Multiprotein Complexes Nuclear Envelope/genetics Sequence Homology, Amino Acid Xenopus Proteins Xenopus laevis
Chemicals
Cell Cycle Proteins Chromatin Chromosomal Proteins, Non-Histone DNA-Binding Proteins Fungal Proteins Macromolecular Substances Multiprotein Complexes SMC1A protein, Xenopus SMC3 protein, Xenopus Xenopus Proteins condensin complexes structural maintenance of chromosome protein 1 Adenosine Triphosphatases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Losada A
Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724 USA.
Hirano M
Hirano T
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35 references, click to expand
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Article Info
Journal
Genes & development
Abbr.
Genes Dev
ISSN
0890-9369
DOI
10.1101/gad.12.13.1986
Published
1998-07-01
Pages
1986-97
Language
English
Region
United States
NLM ID
8711660
PMCID
PMC316973
Subset
IM
Grants
NIGMS NIH HHS · GM53926 · United States
Databases
GENBANK
AF051784, AF051785, AF051786
External Links
PubMed source DOI: 10.1101/gad.12.13.1986 PMC Full Text View journal details
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