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PMID: 9671050 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

Identification of two cis-acting elements that independently regulate the length of poly(A) on Xenopus albumin pre-mRNA.

RNA (New York, N.Y.) ·Vol. 4 ·No. 7 ·1998-07-00 ·Pages 766-76

Das Gupta J, Gu H, Chernokalskaya E, Gao X, Schoenberg DR

Abstract

Unlike most eukaryotic mRNAs studied to date, Xenopus serum albumin mRNA has a short (17-residue), discrete poly(A) tail. We recently reported that this short poly(A) tail results from regulation of the length of poly(A) on albumin pre-mRNA. The purpose of the present study was to locate the cis-acting element responsible for this, the poly(A)-limiting element or PLE. An albumin minigene consisting of albumin cDNA joined in exon 13 to the 3' end of the albumin gene produced mRNA with <20 nt poly(A) when transfected into mouse fibroblasts. This result indicates both that cis-acting sequences that regulate poly(A) length are within this construct, and that nuclear regulation of poly(A) length is conserved between vertebrates. Poly(A) length regulation was retained after replacing the terminal 53 bp and 3' flanking region of the albumin gene with a synthetic polyadenylation element (SPA). Conversely, fusing albumin gene sequence spanning the terminal 53 bp of the albumin gene and 3' flanking sequence onto the human beta-globin gene yielded globin mRNA with a 200-residue poly(A)tail. These data indicate that the PLE resides upstream of the sequence elements involved in albumin pre-mRNA 3' processing. Poly(A) length regulation was restored upon fusing a segment bearing albumin intron 14, exon 15, and 3' flanking sequence onto the beta-globin gene. We demonstrate that exon 15 contains two PLEs that can act independently to regulate the length of poly(A).

MeSH Terms
Animals Base Sequence Exons Humans Mice Molecular Sequence Data Poly A/biosynthesis RNA Precursors/biosynthesis RNA Processing, Post-Transcriptional RNA, Messenger/biosynthesis Recombinant Proteins Regulatory Sequences, Nucleic Acid Serum Albumin/genetics Species Specificity Xenopus
Chemicals
RNA Precursors RNA, Messenger Recombinant Proteins Serum Albumin Poly A
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Das Gupta J
Department of Pharmacology and Comprehensive Cancer Center, The Ohio State University College of Medicine, Columbus 43210-1239, USA.
Gu H
Chernokalskaya E
Gao X
Schoenberg D R
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Article Info
Journal
RNA (New York, N.Y.)
Abbr.
RNA
ISSN
1355-8382
DOI
10.1017/s1355838298971837
Published
1998-07-00
Pages
766-76
Language
English
Region
United States
NLM ID
9509184
PMCID
PMC1369657
Subset
IM
Grants
NIGMS NIH HHS · GM38277 · United States
NIGMS NIH HHS · GM55407 · United States
NCI NIH HHS · P30 CA16058 · United States
External Links
PubMed source DOI: 10.1017/s1355838298971837 PMC Full Text View journal details
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