Some properties of tobacco protoplast chromatin.

Nucleic acids research ·Vol. 3 ·No. 9 ·1976-09-00 ·Pages 2315-29

Gigot C, Philipps G, Nicolaieff A, Hirth L

Abstract

Chromatin was prepared from tobacco-leaf protoplasts. Its solubility in increasing molarities of NaCl was studied and the structure of the soluble fraction observed by electron microscopy. We demonstrate that in plants, the DNA and histones are associated in beaded structures similar to those called omicron-bodies or nucleosomes in animal chromatin. The nucleosomes were associated with DNA in either compact or extended forms. The compact arrangement was predominant in the fraction solubilized between 0.1 and 0.4 M NaCl. The extended form, present at 0.5 and 0.6 M NaCl. showed DNA filaments of various lengths interspacing the nucleosomes. At these ionic strengths ring structures were present, associated with the DNA. At 0.7 M NaCl and above, only DNA filaments were present, occasionally associated with big rings, and nucleosomes were compoetely dissociated. Free DNA molecules were present at all ionic strengths used. The possible origin and significance of the rings are discussed.

MeSH Terms

Cell Nucleus/metabolism Chromatin/ultrastructure DNA Histones/analysis Micrococcal Nuclease Nucleic Acid Conformation Nucleoproteins Osmolar Concentration Plants, Toxic Protoplasts Sodium Chloride Solubility Tobacco

Chemicals

Chromatin Histones Nucleoproteins Sodium Chloride DNA Micrococcal Nuclease

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Gigot C

Philipps G

Nicolaieff A

Hirth L

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24 references, click to expand

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Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/3.9.2315

Published

1976-09-00

Pages

2315-29

Language

English

Region

England

NLM ID

0411011

PMCID

PMC343087

Subset

External Links

PubMed source DOI: 10.1093/nar/3.9.2315 PMC Full Text View journal details

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Some properties of tobacco protoplast chromatin.

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