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PMID: 9685320 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Parallel radiation hybrid mapping: a powerful tool for high-resolution genomic comparison.

Genome research ·Vol. 8 ·No. 7 ·1998-07-00 ·Pages 731-6

Yang YP, Womack JE

Abstract

Comparative gene mapping in mammals typically involves identification of segments of conserved synteny in diverse genomes. The development of maps that permit comparison of gene order within conserved synteny has not advanced beyond the mouse map that takes advantage of linkage analysis in interspecific backcrosses. Radiation hybrid (RH) mapping provides a powerful tool for determining order of genes in genomes for which gene-based linkage mapping is impractical. Comparative RH mapping of 24 orthologous genes in this study revealed internal structural rearrangements between human chromosome 17 (HSA17) and bovine chromosome 19 (BTA19), two chromosomes known previously to be conserved completely and exclusively at level of synteny. Only six of the 24 genes had been previously ordered on the human G3 RH map. The use of the G3 panel to map the other 18, however, produced parallel RH maps for comparison of gene order at a resolution of <5 Mb on the bovine linkage map and from 1 to 3 Mb in the human physical map.

MeSH Terms
Animals Cattle Chromosome Mapping/methods Chromosomes, Human, Pair 17/genetics Genetic Markers Genomic Library Humans Hybrid Cells/metabolism,radiation effects Translocation, Genetic
Chemicals
Genetic Markers
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Yang Y P
Department of Veterinary Pathobiology and Center for Animal Genetics, Institute of Biosciences and Technology, Texas A&M University, College Station, Texas 77843-4467 USA.
Womack J E
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Article Info
Journal
Genome research
Abbr.
Genome Res
ISSN
1088-9051
DOI
10.1101/gr.8.7.731
Published
1998-07-00
Pages
731-6
Language
English
Region
United States
NLM ID
9518021
PMCID
PMC310752
Subset
IM
External Links
PubMed source DOI: 10.1101/gr.8.7.731 PMC Full Text View journal details
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