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PMID: 9687063 Published · ppublish English Journal Article

Identification by PCR of receptor-like protein kinases from Arabidopsis flowers.

Plant molecular biology ·Vol. 37 ·No. 4 ·1998-07-00 ·Pages 587-96

Takahashi T, Mu JH, Gasch A, Chua NH

Abstract

We have isolated three receptor-like kinase cDNAs from an Arabidopsis flower cDNA library by PCR using degenerate oligonucleotide primers for conserved domains of protein kinases. Cloning and sequencing of the full-length cDNAs, designated RKF1 to 3 (receptor-like kinase in flowers), showed that the putative extracellular domain of the RKF1 protein contains 13 tandem repeats of leucine-rich sequences and those of RKF2 and RKF3 have no significant homology with other plant sequences. RNA blot analysis revealed that the RKF1 mRNA is highly expressed in stamens while RKF2 and RKF3 mRNAs are present at low levels in all organs examined. In situ localization experiments indicated that the RKF1 mRNA is detectable in early flower primordia and during stamen development. In addition, when fused to a GUS reporter gene, the RKF1 promoter directed high GUS expression in pollen grains. Recombinant RKF1, produced in Escherichia coli, was found to have kinase activity with serine/threonine specificity in vitro.

MeSH Terms
Amino Acid Sequence Arabidopsis/enzymology,genetics,metabolism Base Sequence Cloning, Molecular DNA Primers/genetics DNA, Complementary/genetics DNA, Plant/genetics Gene Expression Genes, Plant Molecular Sequence Data Plant Proteins/genetics,metabolism Plant Structures/enzymology Polymerase Chain Reaction Protein Kinases/genetics,metabolism RNA, Messenger/genetics,metabolism RNA, Plant/genetics,metabolism Sequence Homology, Amino Acid Tissue Distribution
Chemicals
DNA Primers DNA, Complementary DNA, Plant Plant Proteins RNA, Messenger RNA, Plant Protein Kinases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Takahashi T
Laboratory of Plant Molecular Biology, The Rockefeller University, New York, NY 10021, USA.
Mu J H
Gasch A
Chua N H
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Article Info
Journal
Plant molecular biology
Abbr.
Plant Mol Biol
ISSN
0167-4412
DOI
10.1023/a:1005924817190
Published
1998-07-00
Pages
587-96
Language
English
Region
Netherlands
NLM ID
9106343
Subset
IM
Databases
GENBANK
AF024648, AF024649, AF024650
External Links
PubMed source DOI: 10.1023/a:1005924817190 View journal details
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