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PMID: 9705962 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

SLP-65: a new signaling component in B lymphocytes which requires expression of the antigen receptor for phosphorylation.

The Journal of experimental medicine ·Vol. 188 ·No. 4 ·1998-08-17 ·Pages 791-5

Wienands J, Schweikert J, Wollscheid B, Jumaa H, Nielsen PJ, Reth M

Abstract

The B cell antigen receptor (BCR) consists of the membrane-bound immunoglobulin (Ig) molecule as antigen-binding subunit and the Ig-alpha/Ig-beta heterodimer as signaling subunit. BCR signal transduction involves activation of protein tyrosine kinases (PTKs) and phosphorylation of several proteins, only some of which have been identified. The phosphorylation of these proteins can be induced by exposure of B cells either to antigen or to the tyrosine phosphatase inhibitor pervanadate/H2O2. One of the earliest substrates in B cells is a 65-kD protein, which we identify here as a B cell adaptor protein. This protein, named SLP-65, is part of a signaling complex involving Grb-2 and Vav and shows homology to SLP-76, a signaling element of the T cell receptor. In pervanadate/H2O2-stimulated cells, SLP-65 becomes phosphorylated only upon expression of the BCR. These data suggest that SLP-65 is part of a BCR transducer complex.

MeSH Terms
Adaptor Proteins, Signal Transducing Amino Acid Sequence Animals B-Lymphocytes/drug effects,metabolism Base Sequence Carrier Proteins Cell Cycle Proteins DNA, Complementary Enzyme Inhibitors/pharmacology GRB2 Adaptor Protein Humans Hydrogen Peroxide/pharmacology Immunoglobulin delta-Chains/metabolism Immunoglobulin mu-Chains/metabolism Molecular Sequence Data Phosphoproteins/genetics,metabolism Protein-Tyrosine Kinases/metabolism Proteins/metabolism Proto-Oncogene Proteins/metabolism Proto-Oncogene Proteins c-vav Rabbits Receptors, Antigen, B-Cell/metabolism Signal Transduction Tumor Cells, Cultured Vanadates/pharmacology
Chemicals
Adaptor Proteins, Signal Transducing B cell linker protein Carrier Proteins Cell Cycle Proteins DNA, Complementary Enzyme Inhibitors GRB2 Adaptor Protein GRB2 protein, human Immunoglobulin delta-Chains Immunoglobulin mu-Chains Phosphoproteins Proteins Proto-Oncogene Proteins Proto-Oncogene Proteins c-vav Receptors, Antigen, B-Cell VAV1 protein, human pervanadate Vanadates Hydrogen Peroxide Protein-Tyrosine Kinases
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Wienands J
Department for Molecular Immunology, Biology III, University of Freiburg, 79104 Freiburg, Germany. [email protected]
Schweikert J
Wollscheid B
Jumaa H
Nielsen P J
Reth M
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.188.4.791
Email
[email protected]
Published
1998-08-17
Pages
791-5
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2213353
Subset
IM
Databases
GENBANK
Y17159
External Links
PubMed source DOI: 10.1084/jem.188.4.791 PMC Full Text View journal details
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