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PMID: 9305649 Published · ppublish English Journal Article

The splicing factor SRp20 modifies splicing of its own mRNA and ASF/SF2 antagonizes this regulation.

The EMBO journal ·Vol. 16 ·No. 16 ·1997-08-15 ·Pages 5077-85

Jumaa H, Nielsen PJ

Abstract

SRp20 is a member of the highly conserved SR family of splicing regulators. Using a variety of reporter gene constructs, we show that SRp20 regulates alternative splicing of its own mRNA. Overexpression of SRp20 results in a reduction in the level of exon 4-skipped SRp20 transcripts and activates the production of transcripts containing exon 4. These exon 4-included transcripts encode a truncated protein lacking the C-terminal RS domain. We provide evidence that SRp20 probably enhances the recognition of the otherwise unused, weak splice acceptor of exon 4. The recognition of exons with weak splice acceptor sites may be a general activity of SRp20. Unexpectedly, ASF/SF2, another member of the SR family, antagonizes the effect of SRp20 on SRp20 pre-mRNA splicing and suppresses the production of the exon 4-included form. Our results indicate that ASF/SF2 suppresses the use of the alternative exon 4, most likely by inhibiting the recognition of the splice donor of exon 4. These results demonstrate, for the first time, an auto-regulatory activity of an SR protein which is antagonized by a second SR protein.

MeSH Terms
Alternative Splicing/genetics Animals Base Sequence Blotting, Northern Blotting, Western Cloning, Molecular Exons/genetics Gene Expression Regulation/genetics Genes, Reporter/genetics Genetic Vectors Mice Molecular Sequence Data Mutagenesis, Site-Directed/genetics Nuclear Proteins/physiology Promoter Regions, Genetic/genetics RNA Precursors/genetics,metabolism RNA, Messenger/metabolism RNA-Binding Proteins/antagonists & inhibitors,genetics,metabolism Sequence Analysis, DNA Serine-Arginine Splicing Factors Spliceosomes/chemistry,metabolism Transfection/genetics Tumor Cells, Cultured
Chemicals
Nuclear Proteins RNA Precursors RNA, Messenger RNA-Binding Proteins SRSF3 protein, human Srsf3 protein, mouse Serine-Arginine Splicing Factors
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Jumaa H
Max Planck Institute for Immunobiology, Freiburg, Germany.
Nielsen P J
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
DOI
10.1093/emboj/16.16.5077
Published
1997-08-15
Pages
5077-85
Language
English
Region
England
NLM ID
8208664
PMCID
PMC1170142
Subset
IM
External Links
PubMed source DOI: 10.1093/emboj/16.16.5077 PMC Full Text View journal details
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