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PMID: 97221 Published · ppublish English Journal Article

Immunogenic capacity of ribosomes of Salmonella typhi interfered with a flagellin-like material contaminant.

Infection and immunity ·Vol. 20 ·No. 1 ·1978-04-00 ·Pages 161-6

Cofré G, Calderón I, Mora GC

Abstract

The double-immunodiffusion technique and sodium dodecyl sulfate-polyacrylamide electrophoresis were used to demonstrate the presence of flagellin-like material strongly attached to ribosomes of Salmonella typhi Ty 2. This flagellin-like material contaminating the ribosome preparation interferes with the induction of antiribosome serum promoting the formation of antisera reacting either only with flagellin or in some cases with flagellin and ribosomes, but giving a very weak reaction with the latter. The interference is also observed when purified ribosomes from a nonflagellated mutant of S. typhi (S. typhi O-901) mixed with purified S. typhi Ty 2 flagellin are utilized as antigens. The antiribosome sera obtained with ribosomes from S. typhi O-901 have a considerably higher titer than those that are interfered with. These sera were able to react with ribosomes obtained from several related species and did not react with flagella-derived flagellin of S. typhi Ty 2.

MeSH Terms
Antigens, Bacterial Bacterial Proteins/immunology Cross Reactions Electrophoresis, Polyacrylamide Gel Flagellin/immunology,isolation & purification Immunodiffusion Ribosomes/immunology Salmonella typhi/immunology Sodium Dodecyl Sulfate Species Specificity
Chemicals
Antigens, Bacterial Bacterial Proteins Flagellin Sodium Dodecyl Sulfate
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Cofré G
Calderón I
Mora G C
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30 references, click to expand
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.20.1.161-166.1978
Published
1978-04-00
Pages
161-6
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC421567
Subset
IM
External Links
PubMed source DOI: 10.1128/iai.20.1.161-166.1978 PMC Full Text View journal details
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