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PMID: 9786949 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Identification of Kel1p, a kelch domain-containing protein involved in cell fusion and morphology in Saccharomyces cerevisiae.

The Journal of cell biology ·Vol. 143 ·No. 2 ·1998-10-19 ·Pages 375-89

Philips J, Herskowitz I

Abstract

We showed previously that protein kinase C, which is required to maintain cell integrity, negatively regulates cell fusion (Philips, J., and I. Herskowitz. 1997. J. Cell Biol. 138:961-974). To identify additional genes involved in cell fusion, we looked for genes whose overexpression relieved the defect caused by activated alleles of Pkc1p. This strategy led to the identification of a novel gene, KEL1, which encodes a protein composed of two domains, one containing six kelch repeats, a motif initially described in the Drosophila protein Kelch (Xue, F., and L. Cooley. 1993. Cell. 72:681- 693), and another domain predicted to form coiled coils. Overexpression of KEL1 also suppressed the defect in cell fusion of spa2Delta and fps1Delta mutants. KEL2, which corresponds to ORF YGR238c, encodes a protein highly similar to Kel1p. Its overexpression also suppressed the mating defect associated with activated Pkc1p. Mutants lacking KEL1 exhibited a moderate defect in cell fusion that was exacerbated by activated alleles of Pkc1p or loss of FUS1, FUS2, or FPS1, but not by loss of SPA2. kel1Delta mutants form cells that are elongated and heterogeneous in shape, indicating that Kel1p is also required for proper morphology during vegetative growth. In contrast, kel2Delta mutants were not impaired in cell fusion or morphology. Both Kel1p and Kel2p localized to the site where cell fusion occurs during mating and to regions of polarized growth during vegetative growth. Coimmunoprecipitation and two-hybrid analyses indicated that Kel1p and Kel2p physically interact. We conclude that Kel1p has a role in cell morphogenesis and cell fusion and may antagonize the Pkc1p pathway.

MeSH Terms
Adaptor Proteins, Signal Transducing Carrier Proteins/chemistry,genetics Cell Division/physiology Cell Polarity/physiology Cloning, Molecular Fungal Proteins/chemistry,genetics Gene Expression Regulation, Fungal Membrane Fusion/physiology Molecular Sequence Data Mutation/physiology Protein Structure, Tertiary Reproduction/physiology Saccharomyces cerevisiae/chemistry,cytology,physiology Saccharomyces cerevisiae Proteins Sequence Homology, Amino Acid
Chemicals
Adaptor Proteins, Signal Transducing Carrier Proteins Fungal Proteins KEL1 protein, S cerevisiae KEL2 protein, S cerevisiae Saccharomyces cerevisiae Proteins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Philips J
Department of Biochemistry and Biophysics, University of California, San Francisco, California 94143-0448, USA. [email protected]
Herskowitz I
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.143.2.375
Email
[email protected]
Published
1998-10-19
Pages
375-89
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2132843
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.143.2.375 PMC Full Text View journal details
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