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PMID: 9826724 Published · ppublish English Journal Article

Cellular gene expression altered by human cytomegalovirus: global monitoring with oligonucleotide arrays.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 95 ·No. 24 ·1998-11-24 ·Pages 14470-5

Zhu H, Cong JP, Mamtora G, Gingeras T, Shenk T

Abstract

Mechanistic insights to viral replication and pathogenesis generally have come from the analysis of viral gene products, either by studying their biochemical activities and interactions individually or by creating mutant viruses and analyzing their phenotype. Now it is possible to identify and catalog the host cell genes whose mRNA levels change in response to a pathogen. We have used DNA array technology to monitor the level of approximately 6,600 human mRNAs in uninfected as compared with human cytomegalovirus-infected cells. The level of 258 mRNAs changed by a factor of 4 or more before the onset of viral DNA replication. Several of these mRNAs encode gene products that might play key roles in virus-induced pathogenesis, identifying them as intriguing targets for further study.

MeSH Terms
Annexin A1/genetics Base Sequence Cells, Cultured Cyclooxygenase 2 Cytomegalovirus/physiology DNA/genetics DNA-Binding Proteins/genetics Fibroblasts/metabolism,virology Gene Expression Regulation HLA Antigens/genetics Humans Isoenzymes/genetics Male Membrane Proteins Microphthalmia-Associated Transcription Factor Oligodeoxyribonucleotides Phospholipases A/genetics Promoter Regions, Genetic Prostaglandin-Endoperoxide Synthases/genetics RNA, Messenger/biosynthesis Ribonucleoproteins/genetics Skin/cytology,metabolism,virology Thrombospondins/genetics Transcription Factors/genetics Transcription, Genetic
Chemicals
Annexin A1 DNA-Binding Proteins HLA Antigens Isoenzymes MITF protein, human Membrane Proteins Microphthalmia-Associated Transcription Factor Oligodeoxyribonucleotides RNA, Messenger Ribonucleoproteins Thrombospondins Transcription Factors DNA Cyclooxygenase 2 PTGS2 protein, human Prostaglandin-Endoperoxide Synthases Phospholipases A
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Zhu H
Howard Hughes Medical Institute, Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA.
Cong J P
Mamtora G
Gingeras T
Shenk T
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.95.24.14470
Published
1998-11-24
Pages
14470-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC24397
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.95.24.14470 PMC Full Text View journal details
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