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PMID: 9841922 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

2B4, the natural killer and T cell immunoglobulin superfamily surface protein, is a ligand for CD48.

The Journal of experimental medicine ·Vol. 188 ·No. 11 ·1998-12-07 ·Pages 2083-90

Brown MH, Boles K, van der Merwe PA, Kumar V, Mathew PA, Barclay AN

Abstract

2B4 is a cell surface glycoprotein related to CD2 and implicated in the regulation of natural killer and T lymphocyte function. A recombinant protein containing the extracellular region of mouse (m)2B4 attached to avidin-coated fluorescent beads bound to rodent cells, and binding was completely blocked by CD48 monoclonal antibodies (mAbs). Using surface plasmon resonance, we showed that purified soluble mCD48 bound m2B4 with a six- to ninefold higher affinity (Kd approximately 16 microM at 37 degreesC) than its other ligand, CD2. Human CD48 bound human 2B4 with a similar affinity (Kd approximately 8 microM). The finding of an additional ligand for CD48 provides an explanation for distinct functional effects observed on perturbing CD2 and CD48 with mAbs or by genetic manipulation.

MeSH Terms
Animals Antigens, CD/immunology CD48 Antigen Flow Cytometry Humans Immunoglobulins/immunology Killer Cells, Natural/immunology Ligands Membrane Glycoproteins/immunology Mice Receptors, Immunologic Signaling Lymphocytic Activation Molecule Family T-Lymphocytes/immunology
Chemicals
Antigens, CD CD244 protein, human CD48 Antigen CD48 protein, human Cd244a protein, mouse Cd48 protein, mouse Immunoglobulins Ligands Membrane Glycoproteins Receptors, Immunologic Signaling Lymphocytic Activation Molecule Family
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Brown M H
Medical Research Council Cellular Immunology Unit, Sir William Dunn School of Pathology, University of Oxford, Oxford OX1 3RE, United Kingdom.
Boles K
van der Merwe P A
Kumar V
Mathew P A
Barclay A N
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.188.11.2083
Published
1998-12-07
Pages
2083-90
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2212392
Subset
IM
External Links
PubMed source DOI: 10.1084/jem.188.11.2083 PMC Full Text View journal details
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