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PMID: 9843577 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

The cdr2(+) gene encodes a regulator of G2/M progression and cytokinesis in Schizosaccharomyces pombe.

Molecular biology of the cell ·Vol. 9 ·No. 12 ·1998-12-00 ·Pages 3399-415

Breeding CS, Hudson J, Balasubramanian MK, Hemmingsen SM, Young PG, Gould KL

Abstract

Schizosaccharomyces pombe cells respond to nutrient deprivation by altering G2/M cell size control. The G2/M transition is controlled by activation of the cyclin-dependent kinase Cdc2p. Cdc2p activation is regulated both positively and negatively. cdr2(+) was identified in a screen for regulators of mitotic control during nutrient deprivation. We have cloned cdr2(+) and have found that it encodes a putative serine-threonine protein kinase that is related to Saccharomyces cerevisiae Gin4p and S. pombe Cdr1p/Nim1p. cdr2(+) is not essential for viability, but cells lacking cdr2(+) are elongated relative to wild-type cells, spending a longer period of time in G2. Because of this property, upon nitrogen deprivation cdr2(+) mutants do not arrest in G1, but rather undergo another round of S phase and arrest in G2 from which they are able to enter a state of quiescence. Genetic evidence suggests that cdr2(+) acts as a mitotic inducer, functioning through wee1(+), and is also important for the completion of cytokinesis at 36 degrees C. Defects in cytokinesis are also generated by the overproduction of Cdr2p, but these defects are independent of wee1(+), suggesting that cdr2(+) encodes a second activity involved in cytokinesis.

MeSH Terms
ATP-Binding Cassette Transporters/genetics,physiology Amino Acid Sequence Base Sequence Cell Division/genetics,physiology DNA Primers/genetics DNA, Fungal/genetics Fungal Proteins/genetics,physiology G2 Phase/genetics,physiology Gene Expression Genes, Fungal Mitosis/genetics,physiology Molecular Sequence Data Mutation Nitrogen/metabolism Protein Serine-Threonine Kinases/genetics,physiology Schizosaccharomyces/cytology,genetics Sequence Homology, Amino Acid
Chemicals
ATP-Binding Cassette Transporters DNA Primers DNA, Fungal Fungal Proteins Protein Serine-Threonine Kinases Nitrogen
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Breeding C S
Department of Cell Biology, Vanderbilt University, Nashville, Tennessee, 37212, USA.
Hudson J
Balasubramanian M K
Hemmingsen S M
Young P G
Gould K L
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Article Info
Journal
Molecular biology of the cell
Abbr.
Mol Biol Cell
ISSN
1059-1524
DOI
10.1091/mbc.9.12.3399
Published
1998-12-00
Pages
3399-415
Language
English
Region
United States
NLM ID
9201390
PMCID
PMC25645
Subset
IM
Grants
NIGMS NIH HHS · GM-47728 · United States
NCI NIH HHS · T32 CA-09592 · United States
External Links
PubMed source DOI: 10.1091/mbc.9.12.3399 PMC Full Text View journal details
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