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PMID: 9851980 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Drosophila Cyclin B3 is required for female fertility and is dispensable for mitosis like Cyclin B.

Genes & development ·Vol. 12 ·No. 23 ·1998-12-01 ·Pages 3741-51

Jacobs HW, Knoblich JA, Lehner CF

Abstract

Cyclin B3 has been conserved during higher eukaryote evolution as evidenced by its identification in chicken, nematodes, and insects. We demonstrate that Cyclin B3 is present in addition to Cyclins A and B in mitotically proliferating cells and not detectable in endoreduplicating tissues of Drosophila embryos. Cyclin B3 is coimmunoprecipitated with Cdk1(Cdc2) but not with Cdk2(Cdc2c). It is degraded abruptly during mitosis like Cyclins A and B. In contrast to these latter cyclins, which accumulate predominantly in the cytoplasm during interphase, Cyclin B3 is a nuclear protein. Genetic analyses indicate functional redundancies. Double and triple mutant analyses demonstrate that Cyclins A, B, and B3 cooperate to regulate mitosis, but surprisingly single mutants reveal that neither Cyclin B3 nor Cyclin B is required for mitosis. However, both are required for female fertility and Cyclin B also for male fertility.

MeSH Terms
Amino Acid Sequence Animals CDC2 Protein Kinase/metabolism Cell Compartmentation Cell Nucleus/metabolism Cyclin A/genetics,metabolism Cyclin B/genetics,metabolism Drosophila/embryology Drosophila Proteins Female Fertility Male Mitosis Molecular Sequence Data Mutation Nuclear Proteins/genetics,metabolism Ovum/metabolism Protein Binding Sequence Homology, Amino Acid
Chemicals
CycB protein, Drosophila Cyclin A Cyclin B Drosophila Proteins Nuclear Proteins CDC2 Protein Kinase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Jacobs H W
Department of Genetics, University of Bayreuth, 95440 Bayreuth, Germany.
Knoblich J A
Lehner C F
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Article Info
Journal
Genes & development
Abbr.
Genes Dev
ISSN
0890-9369
DOI
10.1101/gad.12.23.3741
Published
1998-12-01
Pages
3741-51
Language
English
Region
United States
NLM ID
8711660
PMCID
PMC317254
Subset
IM
Databases
GENBANK
AJ006772, AJ006773, AJ012568
External Links
PubMed source DOI: 10.1101/gad.12.23.3741 PMC Full Text View journal details
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