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PMID: 9852001 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Cytochrome bd biosynthesis in Bacillus subtilis: characterization of the cydABCD operon.

Journal of bacteriology ·Vol. 180 ·No. 24 ·1998-12-00 ·Pages 6571-80

Winstedt L, Yoshida K, Fujita Y, von Wachenfeldt C

Abstract

Under aerobic conditions Bacillus subtilis utilizes a branched electron transport chain comprising various cytochromes and terminal oxidases. At present there is evidence for three types of terminal oxidases in B. subtilis: a caa3-, an aa3-, and a bd-type oxidase. We report here the cloning of the structural genes (cydA and cydB) encoding the cytochrome bd complex. Downstream of the structural genes, cydC and cydD are located. These genes encode proteins showing similarity to bacterial ATP-binding cassette (ABC)-type transporters. Analysis of isolated cell membranes showed that inactivation of cydA or deletion of cydABCD resulted in the loss of spectral features associated with cytochrome bd. Gene disruption experiments and complementation analysis showed that the cydC and cydD gene products are required for the expression of a functional cytochrome bd complex. Disruption of the cyd genes had no apparent effect on the growth of cells in broth or defined media. The expression of the cydABCD operon was investigated by Northern blot analysis and by transcriptional and translational cyd-lacZ fusions. Northern blot analysis confirmed that cydABCD is transcribed as a polycistronic message. The operon was found to be expressed maximally under conditions of low oxygen tension.

MeSH Terms
Bacillus subtilis/enzymology,genetics Base Sequence Cytochrome b Group Cytochromes/biosynthesis,genetics DNA, Bacterial Electron Transport Chain Complex Proteins Escherichia coli Proteins Gene Expression Molecular Sequence Data Multigene Family Mutagenesis Operon Oxidoreductases/biosynthesis,genetics RNA, Bacterial Transcription, Genetic
Chemicals
Cytochrome b Group Cytochromes DNA, Bacterial Electron Transport Chain Complex Proteins Escherichia coli Proteins RNA, Bacterial Oxidoreductases cytochrome bd terminal oxidase complex, E coli
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Winstedt L
Department of Microbiology, Lund University, Lund, Sweden.
Yoshida K
Fujita Y
von Wachenfeldt C
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/JB.180.24.6571-6580.1998
Published
1998-12-00
Pages
6571-80
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC107760
Subset
IM
External Links
PubMed source DOI: 10.1128/JB.180.24.6571-6580.1998 PMC Full Text View journal details
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