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PMID: 9854092 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Comparison of two PCRs for detection of Mycobacterium ulcerans.

Journal of clinical microbiology ·Vol. 37 ·No. 1 ·1999-01-00 ·Pages 206-8

Guimaraes-Peres A, Portaels F, de Rijk P, Fissette K, Pattyn SR, van Vooren J, Fonteyne P

Abstract

Two nested PCRs for the detection of Mycobacterium ulcerans were compared by using a collection of 65 clinical specimens. The first method amplifies the gene coding for 16S rRNA, and the second method amplifies a repetitive DNA sequence. The sensitivities of bacterioscopy, culture, 16S rRNA gene PCR, and repetitive-sequence PCR were 29, 34, 80, and 85%, respectively. Compared to the 16S rRNA gene PCR, the repetitive-sequence PCR was faster, easier to perform, and less expensive.

MeSH Terms

Bacteriological Techniques DNA Transposable Elements/genetics DNA, Bacterial/analysis,genetics Humans Mycobacterium ulcerans/genetics,isolation & purification Polymerase Chain Reaction/methods RNA, Ribosomal, 16S/analysis,genetics Sensitivity and Specificity

Chemicals

DNA Transposable Elements DNA, Bacterial RNA, Ribosomal, 16S

Authors & Affiliations

7 authors, click to expand affiliations / ORCID

Guimaraes-Peres A

Mycobacteriology Unit, Institute of Tropical Medicine, Antwerp, Belgium.

Portaels F

de Rijk P

Fissette K

Pattyn S R

van Vooren J

Fonteyne P

References (14)

14 references, click to expand

[Bacteriology and human and experimental pathology of ulcers caused by Mycobacterium ulcerans].
Ann Soc Belges Med Trop Parasitol Mycol. 1965;45(2):121-9 PMID: 5829986
A method for counting acid-fast bacteria.
Int J Lepr Other Mycobact Dis. 1968 Jan-Mar;36(1):78-82 PMID: 4869698
A new Mycobacterium which caused skin infection.
Microbiol Immunol. 1982;26(10):951-5 PMID: 7162435
Isolation of Mycobacterium paratuberculosis from intestinal mucosa and mesenteric lymph nodes of goats by use of selective Dubos medium.
J Clin Microbiol. 1985 Aug;22(2):312-3 PMID: 3897274
Selective isolation of mycobacteria from soil: a statistical analysis approach.
J Gen Microbiol. 1988 Mar;134(3):849-55 PMID: 3183623
Proposed minimal standards for the genus Mycobacterium and for description of new slowly growing Mycobacterium species.
Int J Syst Bacteriol. 1992 Apr;42(2):315-23 PMID: 1581193
[Mycobacterium ulcerans cutaneous infections: Buruli ulcers].
Med Trop (Mars). 1995;55(4):363-73 PMID: 8830223
Mycobacterium ulcerans infection (Buruli ulcer): first reported patients in Togo.
Br J Dermatol. 1996 Jun;134(6):1116-21 PMID: 8763437
Direct detection and identification of Mycobacterium ulcerans in clinical specimens by PCR and oligonucleotide-specific capture plate hybridization.
J Clin Microbiol. 1997 May;35(5):1097-100 PMID: 9114387
Development of a PCR assay for rapid diagnosis of Mycobacterium ulcerans infection.
J Clin Microbiol. 1997 Jul;35(7):1696-700 PMID: 9196176
Immunomagnetic separation and PCR for detection of Mycobacterium ulcerans.
J Clin Microbiol. 1997 Oct;35(10):2709-11 PMID: 9316944
Detection of Mycobacterium ulcerans in environmental samples during an outbreak of ulcerative disease.
Appl Environ Microbiol. 1997 Oct;63(10):4135-8 PMID: 9327583
Effects of decontamination methods and culture conditions on viability of Mycobacterium ulcerans in the BACTEC system.
J Clin Microbiol. 1998 Feb;36(2):402-8 PMID: 9466749
A new mycobacterial infection in man.
J Pathol Bacteriol. 1948 Jan;60(1):93-122 PMID: 18876541

Article Info

Journal

Journal of clinical microbiology

Abbr.

J Clin Microbiol

ISSN

0095-1137

DOI

10.1128/JCM.37.1.206-208.1999

Published

1999-01-00

Pages

206-8

Language

English

Region

United States

NLM ID

7505564

PMCID

PMC84208

Subset

External Links

PubMed source DOI: 10.1128/JCM.37.1.206-208.1999 PMC Full Text View journal details

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Comparison of two PCRs for detection of Mycobacterium ulcerans.

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