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PMID: 9860980 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Differential regulation of transcription: repression by unactivated mitogen-activated protein kinase Kss1 requires the Dig1 and Dig2 proteins.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 95 ·No. 26 ·1998-12-22 ·Pages 15400-5

Bardwell L, Cook JG, Zhu-Shimoni JX, Voora D, Thorner J

Abstract

Kss1, a yeast mitogen-activated protein kinase (MAPK), in its unphosphorylated (unactivated) state binds directly to and represses Ste12, a transcription factor necessary for expression of genes whose promoters contain filamentous response elements (FREs) and genes whose promoters contain pheromone response elements (PREs). Herein we show that two nuclear proteins, Dig1 and Dig2, are required cofactors in Kss1-imposed repression. Dig1 and Dig2 cooperate with Kss1 to repress Ste12 action at FREs and regulate invasive growth in a naturally invasive strain. Kss1-imposed Dig-dependent repression of Ste12 also occurs at PREs. However, maintenance of repression at PREs is more dependent on Dig1 and/or Dig2 and less dependent on Kss1 than repression at FREs. In addition, derepression at PREs is more dependent on MAPK-mediated phosphorylation than is derepression at FREs. Differential utilization of two types of MAPK-mediated regulation (binding-imposed repression and phosphorylation-dependent activation), in combination with distinct Ste12-containing complexes, contributes to the mechanisms by which separate extracellular stimuli that use the same MAPK cascade can elicit two different transcriptional responses.

MeSH Terms
Calcium-Calmodulin-Dependent Protein Kinases/metabolism Fungal Proteins/genetics,metabolism Gene Expression Regulation, Fungal Genes, Reporter Genotype Mitogen-Activated Protein Kinases Models, Biological Pheromones/physiology Phosphorylation Promoter Regions, Genetic Recombinant Proteins/metabolism Saccharomyces cerevisiae/genetics,growth & development,metabolism Saccharomyces cerevisiae Proteins Transcription Factors/genetics,metabolism Transcription, Genetic
Chemicals
DIG1 protein, S cerevisiae DIG2 protein, S cerevisiae Fungal Proteins Pheromones Recombinant Proteins STE12 protein, S cerevisiae Saccharomyces cerevisiae Proteins Transcription Factors Calcium-Calmodulin-Dependent Protein Kinases FUS3 protein, S cerevisiae KSS1 protein, S cerevisiae Mitogen-Activated Protein Kinases
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Bardwell L
Department of Molecular and Cell Biology, Division of Biochemistry and Molecular Biology, University of California, Berkeley, CA 94720, USA.
Cook J G
Zhu-Shimoni J X
Voora D
Thorner J
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.95.26.15400
Published
1998-12-22
Pages
15400-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC28054
Subset
IM
Grants
NIGMS NIH HHS · R01 GM021841 · United States
NCI NIH HHS · CA09041 · United States
NIGMS NIH HHS · F32 GM019474 · United States
NIGMS NIH HHS · GM21841 · United States
NCI NIH HHS · T32 CA009041 · United States
External Links
PubMed source DOI: 10.1073/pnas.95.26.15400 PMC Full Text View journal details
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