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PMID: 8917571 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Signal transduction through homologs of the Ste20p and Ste7p protein kinases can trigger hyphal formation in the pathogenic fungus Candida albicans.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 93 ·No. 23 ·1996-11-12 ·Pages 13217-22

Leberer E, Harcus D, Broadbent ID, Clark KL, Dignard D, Ziegelbauer K, Schmidt A, Gow NA, Brown AJ, Thomas DY

Abstract

The CST20 gene of Candida albicans was cloned by functional complementation of a deletion of the STE20 gene in Saccharomyces cerevisiae. CST20 encodes a homolog of the Ste20p/p65PAK family of protein kinases. Colonies of C. albicans cells deleted for CST20 revealed defects in the lateral formation of mycelia on synthetic solid "Spider" media. However, hyphal development was not impaired in some other media. A similar phenotype was caused by deletion of HST7, encoding a functional homolog of the S. cerevisiae Ste7p protein kinase. Overexpression of HST7 partially complemented the deletion of CST20. Cells deleted for CST20 were less virulent in a mouse model for systemic candidiasis. Our results suggest that more than one signaling pathway can trigger hyphal development in C. albicans, one of which has a protein kinase cascade that is analogous to the mating response pathway in S. cerevisiae and might have become adapted to the control of mycelial formation in asexual C. albicans.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Candida albicans/genetics,pathogenicity,physiology Candidiasis/physiopathology Consensus Sequence DNA Primers Fungal Proteins/genetics,metabolism Gene Deletion Genes, Fungal Genetic Complementation Test Genomic Library Intracellular Signaling Peptides and Proteins MAP Kinase Kinase Kinases Male Mice Mice, Inbred Strains Mitogen-Activated Protein Kinase Kinases Molecular Sequence Data Polymerase Chain Reaction Protein Kinases/biosynthesis,chemistry,genetics,metabolism,physiology Protein Serine-Threonine Kinases/biosynthesis,physiology Saccharomyces cerevisiae/genetics,growth & development Saccharomyces cerevisiae Proteins Sequence Homology, Amino Acid Signal Transduction Virulence
Chemicals
DNA Primers Fungal Proteins Intracellular Signaling Peptides and Proteins Saccharomyces cerevisiae Proteins Protein Kinases CST20 protein, Candida albicans Protein Serine-Threonine Kinases MAP Kinase Kinase Kinases STE20 protein, S cerevisiae HST7 protein, Candida albicans Mitogen-Activated Protein Kinase Kinases STE7 protein, S cerevisiae
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Leberer E
Biotechnology Research Institute, National Research Council of Canada, Montreal, Canada. [email protected]
Harcus D
Broadbent I D
Clark K L
Dignard D
Ziegelbauer K
Schmidt A
Gow N A
Brown A J
Thomas D Y
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.93.23.13217
Email
[email protected]
Published
1996-11-12
Pages
13217-22
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC24073
Subset
IM
Databases
GENBANK
L47210
External Links
PubMed source DOI: 10.1073/pnas.93.23.13217 PMC Full Text View journal details
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